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Biomarkers of nucleic acid oxidation ? A summary state-of-the-art
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SYSNO ASEP 0551701 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biomarkers of nucleic acid oxidation ? A summary state-of-the-art Author(s) Chao, M.R. (GB)
Evans, M.D. (TW)
Hu, Ch.W. (TW)
Ji, Y.H.E. (US)
Moller, P. (DK)
Rössner ml., Pavel (UEM-P) RID, ORCID
Cooke, M.S. (US)Article number 101872 Source Title Redox Biology. - : Elsevier - ISSN 2213-2317
Roč. 42, jun. (2021)Number of pages 22 s. Language eng - English Country NL - Netherlands Keywords Oxidative stress ; DNA ; RNA ; nucleotide pool ; biomarkers ; DNA repair Subject RIV DN - Health Impact of the Environment Quality OECD category Public and environmental health R&D Projects EF16_019/0000798 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEM-P - RVO:68378041 UT WOS 000643849500006 EID SCOPUS 85100819807 DOI 10.1016/j.redox.2021.101872 Annotation Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the other nucleic acids, RNA and the (2?deoxy-)ribonucleotide pools, and evidence is growing that these too may have an important role in disease. LCMS/MS has the ability to provide absolute quantification of specific biomarkers, such as 8-oxo-7,8-dihydro-2?deoxyGuo (8-oxodG), in both nuclear and mitochondrial DNA, and 8-oxoGuo in RNA. However, significant quantities of tissue are needed, limiting its use in human biomonitoring studies. In contrast, the comet assay requires much less material, and as little as 5 ?L of blood may be used, offering a minimally invasive means of assessing oxidative stress in vivo, but this is restricted to nuclear DNA damage only. Urine is an ideal matrix in which to non-invasively study nucleic acid-derived biomarkers of oxidative stress, and considerable progress has been made towards robustly validating these measurements, not least through the efforts of the European Standards Committee on Urinary (DNA) Lesion Analysis. For urine, LC-MS/MS is considered the gold standard approach, and although there have been improvements to the ELISA methodology, this is largely limited to 8oxodG. Emerging DNA adductomics approaches, which either comprehensively assess the totality of adducts in DNA, or map DNA damage across the nuclear and mitochondrial genomes, offer the potential to considerably advance our understanding of the mechanistic role of oxidatively damaged nucleic acids in disease. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S2213231721000203?via%3Dihub
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