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Biomarkers of nucleic acid oxidation ? A summary state-of-the-art

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    SYSNO ASEP0551701
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleBiomarkers of nucleic acid oxidation ? A summary state-of-the-art
    Author(s) Chao, M.R. (GB)
    Evans, M.D. (TW)
    Hu, Ch.W. (TW)
    Ji, Y.H.E. (US)
    Moller, P. (DK)
    Rössner ml., Pavel (UEM-P) RID, ORCID
    Cooke, M.S. (US)
    Article number101872
    Source TitleRedox Biology. - : Elsevier - ISSN 2213-2317
    Roč. 42, jun. (2021)
    Number of pages22 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsOxidative stress ; DNA ; RNA ; nucleotide pool ; biomarkers ; DNA repair
    Subject RIVDN - Health Impact of the Environment Quality
    OECD categoryPublic and environmental health
    R&D ProjectsEF16_019/0000798 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportUEM-P - RVO:68378041
    UT WOS000643849500006
    EID SCOPUS85100819807
    DOI10.1016/j.redox.2021.101872
    AnnotationOxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the other nucleic acids, RNA and the (2?deoxy-)ribonucleotide pools, and evidence is growing that these too may have an important role in disease. LCMS/MS has the ability to provide absolute quantification of specific biomarkers, such as 8-oxo-7,8-dihydro-2?deoxyGuo (8-oxodG), in both nuclear and mitochondrial DNA, and 8-oxoGuo in RNA. However, significant quantities of tissue are needed, limiting its use in human biomonitoring studies. In contrast, the comet assay requires much less material, and as little as 5 ?L of blood may be used, offering a minimally invasive means of assessing oxidative stress in vivo, but this is restricted to nuclear DNA damage only. Urine is an ideal matrix in which to non-invasively study nucleic acid-derived biomarkers of oxidative stress, and considerable progress has been made towards robustly validating these measurements, not least through the efforts of the European Standards Committee on Urinary (DNA) Lesion Analysis. For urine, LC-MS/MS is considered the gold standard approach, and although there have been improvements to the ELISA methodology, this is largely limited to 8oxodG. Emerging DNA adductomics approaches, which either comprehensively assess the totality of adducts in DNA, or map DNA damage across the nuclear and mitochondrial genomes, offer the potential to considerably advance our understanding of the mechanistic role of oxidatively damaged nucleic acids in disease.
    WorkplaceInstitute of Experimental Medicine
    ContactLenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
    Year of Publishing2022
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S2213231721000203?via%3Dihub
Number of the records: 1  

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