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Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage
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SYSNO ASEP 0548228 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage Author(s) Jaime-Perez, Noelia (BC-A)
Bína, David (BC-A) RID, ORCID
Kaftan, David (MBU-M) ORCID
Bokhari, Syed Nadeem Hussain (BC-A) RID
Küpper, Hendrik (BC-A) RID, ORCIDNumber of authors 5 Article number 148472 Source Title Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1862, č. 10 (2021)Number of pages 12 s. Language eng - English Country NL - Netherlands Keywords Bacteriochlorophyll ; Photosynthetic bacteria ; Acidiphilium rubrum ; Rhodospirillum rubrum ; Pigment-protein complexes ; Copper stress ; Reaction centre ; Trans-metallation Subject RIV BO - Biophysics OECD category Biophysics Subject RIV - cooperation Institute of Microbiology - Biochemistry R&D Projects EF15_003/0000336 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-14024S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 ; MBU-M - RVO:61388971 UT WOS 000681042600002 EID SCOPUS 85110290208 DOI 10.1016/j.bbabio.2021.148472 Annotation In almost all photosynthetic organisms the photosynthetic pigments chlorophyll and bacteriochlorophyll (BChl) are Mg2+ containing complexes, but Mg2+ may be exchanged against other metal ions when these are present in toxic concentrations, leading to inactivation of photosynthesis. In this report we studied mechanisms of copper toxicity to the photosynthetic apparatus of Acidiphilium rubrum, an acidophilic purple bacterium that uses Zn2+ instead of Mg2+ as the central metal in the BChl molecules ([Zn]-BChl) of its reaction centres (RCs) and light harvesting proteins (LH1). We used a combination of in vivo measurements of photosynthetic activity (fast fluorescence and absorption kinetics) together with analysis of metal binding to pigments and pigment-protein complexes by HPLC-ICP-sfMS to monitor the effect of Cu2+ on photosynthesis of A. rubrum. Further, we found that its cytoplasmic pH is neutral. We compared these results with those obtained from Rhodospirillum rubrum, a purple bacterium for which we previously reported that the central Mg2+ of BChl can be replaced in vivo in the RCs by Cu2+ under environmentally realistic Cu-2+ concentrations, leading to a strong inhibition of photosynthesis. Thus, we observed that A. rubrum is much more resistant to copper toxicity than R. rubrum. Only slight changes of photosynthetic parameters were observed in A. rubrum at copper concentrations that were severely inhibitory in R. rubrum and in A. rubrum no copper complexes of BChl were found. Altogether, the data suggest that [Zn]-BChl protects the photosynthetic apparatus of A. rubrum from detrimental insertion of Cu2+ (trans-metallation) into BChl molecules of its RCs. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0166445X21000771?via%3Dihub
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