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Droplet microfluidics with photon-upconversion luminiscence detection: Towards automated single-cell assays
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SYSNO ASEP 0547462 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Droplet microfluidics with photon-upconversion luminiscence detection: Towards automated single-cell assays Author(s) Křivánková, Jana (UIACH-O) RID, ORCID
Hlaváček, Antonín (UIACH-O) ORCID
Foret, František (UIACH-O) RID, ORCIDNumber of authors 3 Source Title Book of abstracts of the 11th International Conference Analytical Cytometry, 1st edition. - Praha : AMCA, spol. s r.o., 2021 - ISBN 978-80-88214-26-7
S. 1-1Number of pages 1 s. Publication form Online - E Action International Conference Analytical Cytometry /11./ Event date 02.10.2021 - 05.10.2021 VEvent location Ostrava Country CZ - Czech Republic Event type WRD Language aba - abeština Country CZ - Czech Republic Keywords droplet-based microfluidic chip ; photon-upconversion nanoparticles ; single-molecule detection Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA21-03156S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation Here, a droplet-based microfluidic chip with luminescence photon-upconversion detection was used for indexing the chemical content of microdroplets (Figure 1). The technique is used for the assay of β-glucosidase that hydrolyzes 4-methylumbelliferyl
β-D-glucopyranoside into fluorescent 4-methylumbelliferone. Further, we discuss the applicability of the presented instrumentation for single-cell analysis utilizing either enzymatic or immunochemical methods with capabilities for single-molecule detection.Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022
Number of the records: 1