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Gradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress-Importance of Carotenoid Accumulation
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SYSNO ASEP 0546702 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress-Importance of Carotenoid Accumulation Author(s) Canonico, Myriam (MBU-M) ORCID
Konert, Grzegorz (MBU-M) ORCID
Crepin, Aurelia (MBU-M) ORCID
Šedivá, Barbora (MBU-M)
Kaňa, Radek (MBU-M) RID, ORCIDArticle number 1916 Source Title Cells. - : MDPI
Roč. 10, č. 8 (2021)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords high light ; thylakoid membrane ; microdomains ; carotenoids ; photoprotection ; Synechocystis ; non-photochemical quenching ; photoinhibition ; photosystems Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA19-11494S GA ČR - Czech Science Foundation (CSF) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000688949100001 EID SCOPUS 85115042828 DOI 10.3390/cells10081916 Annotation Light plays an essential role in photosynthesis, however, its excess can cause damage to cellular components. Photosynthetic organisms thus developed a set of photoprotective mechanisms (e.g., non-photochemical quenching, photoinhibition) that can be studied by a classic biochemical and biophysical methods in cell suspension. Here, we combined these bulk methods with single-cell identification of microdomains in thylakoid membrane during high-light (HL) stress. We used Synechocystis sp. PCC 6803 cells with YFP tagged photosystem I. The single-cell data pointed to a three-phase response of cells to acute HL stress. We defined: (1) fast response phase (0-30 min), (2) intermediate phase (30-120 min), and (3) slow acclimation phase (120-360 min). During the first phase, cyanobacterial cells activated photoprotective mechanisms such as photoinhibition and non-photochemical quenching. Later on (during the second phase), we temporarily observed functional decoupling of phycobilisomes and sustained monomerization of photosystem II dimer. Simultaneously, cells also initiated accumulation of carotenoids, especially gamma-carotene, the main precursor of all carotenoids. In the last phase, in addition to gamma-carotene, we also observed accumulation of myxoxanthophyll and more even spatial distribution of photosystems and phycobilisomes between microdomains. We suggest that the overall carotenoid increase during HL stress could be involved either in the direct photoprotection (e.g., in ROS scavenging) and/or could play an additional role in maintaining optimal distribution of photosystems in thylakoid membrane to attain efficient photoprotection. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2073-4409/10/8/1916
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