Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei)

Ferreira, Marco Tulio Mendes; Glombik, Marek; Perničková, Kateřina; Duchoslav, M.; Scholten, O.; Karafiátová, Miroslava; Techio, V.H.; Doležel, Jaroslav; Lukaszewski, A.J.; Kopecký, David

doi:https://dx.doi.org/10.1093/jxb/eraa455

Number of the records: 1

Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei)

1.

SYSNO ASEP	0545209
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei)
Author(s)	Ferreira, Marco Tulio Mendes (UEB-Q)_ORCID Glombik, Marek (UEB-Q)_ORCID Perničková, Kateřina (UEB-Q)_ORCID Duchoslav, M. (CZ) Scholten, O. (NL) Karafiátová, Miroslava (UEB-Q)_{RID, ORCID} Techio, V.H. (BR) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Lukaszewski, A.J. (US) Kopecký, David (UEB-Q)_{RID, ORCID}
Number of authors	10
Source Title	Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957 Roč. 72, č. 2 (2021), s. 254-267
Number of pages	14 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	crossing-over ; chiasma interference ; meiotic recombination ; tetrad analysis ; saccharomyces-cerevisiae ; individual chromosomes ; physical distribution ; statistical-analysis ; festuca-pratensis ; linked genes ; Centromere ; chromatid interference ; crossover interference ; homoeologous chromosome ; hybrid ; meiosis ; recombination
OECD category	Genetics and heredity (medical genetics to be 3)
R&D Projects	GA20-10019S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000648886400010
EID SCOPUS	85102929034
DOI	10.1093/jxb/eraa455
Annotation	Crossing over, in addition to its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% versus 25%). We also provide a physical measure of the crossover interference distance, covering similar to 30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2022
Electronic address	http://doi.org/10.1093/jxb/eraa455

Number of the records: 1