Number of the records: 1
High-performance countercurrent chromatography for lutein production from a chlorophyll-deficient strain of the microalgae Parachlorella kessleri HY1
- 1.
SYSNO ASEP 0544824 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-performance countercurrent chromatography for lutein production from a chlorophyll-deficient strain of the microalgae Parachlorella kessleri HY1 Author(s) Fábryová, Tereza (MBU-M) ORCID
Kubáč, David (MBU-M) RID, ORCID
Kuzma, Marek (MBU-M) ORCID, RID
Hrouzek, Pavel (MBU-M) ORCID
Kopecký, Jiří (MBU-M) ORCID
Tůmová, L. (CZ)
Cheel Horna, José (MBU-M)Source Title Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 33, č. 4 (2021), s. 1999-2013Number of pages 15 s. Language eng - English Country US - United States Keywords Lutein ; Microalgae Parachlorella kessleri ; High-performance countercurrent chromatography (HPCCC) ; Liquid ; liquid chromatography Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects FV10155 GA MPO - Ministry of Industry and Trade (MPO) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000630854500002 EID SCOPUS 85103149747 DOI 10.1007/s10811-021-02434-y Annotation Green microalgae are a recognized lutein source, however, processing for lutein production requires additional operations such as extraction and saponification, mainly due to the high green pigment and lipid content in the biomass. In this study lutein was isolated from a chlorophyll-deficient Parachlorella kessleri HY1 strain using high-performance countercurrent chromatography (HPCCC). The lower phase of the biphasic solvent system composed of n-heptane-ethanol-water, 5:4:1.5, v/v/v was used both as biomass extraction solvent and HPCCC mobile phase conferring a high selectivity to the lutein production process. For the HPCCC isolation, a multiple injection method was developed, and ten consecutive sample injections (300 mg per each) were performed. To favor the economics of the process, the HPCCC mobile and stationary phases were separately formulated based on nuclear magnetic resonance (NMR) analyses. This strategy enabled to avoid obtaining immiscible liquid phases from their parent biphasic solvent system, which led to the reduction of the separation process duration and solvent consumption. Overall, 3 g of P. kessleri HY1 strain extract was processed by HPCCC yielding 150 mg of lutein (95% purity, 97% recovery). The results presented here form an efficient and economical basis for the large-scale production of microalgae-sourced lutein. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://link.springer.com/article/10.1007%2Fs10811-021-02434-y
Number of the records: 1