TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation

0543922 - ÚMG 2022 RIV GB eng J - Journal Article
Klimešová, Klára - Vojáčková, Jitka - Radivojevic, Nenad - Vandermoere, F. - Bertrand, E. - Verheggen, C. - Staněk, David
TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.
Nature Communications. Roč. 12, č. 1 (2021), č. článku 3646. E-ISSN 2041-1723
R&D Projects: GA ČR(CZ) GA18-10035S; GA ČR GA21-04132S; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT LO1419
Institutional support: RVO:68378050
Keywords : spliceosome * RNN splicing * Cajal body * snRNP * chaperone
OECD category: Biochemistry and molecular biology
Impact factor: 17.694, year: 2021
Method of publishing: Open access
https://www.nature.com/articles/s41467-021-23934-y

U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6 center dot U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leaves as a U5/PRPF19 post-splicing particle, which requires re-generation before the next round of splicing. Here, we show that a previously uncharacterized protein TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. We provide evidence that TSSC4 associates with U5 snRNP chaperones, U5 snRNP and the U5/PRPF19 particle. Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200. We also identified TSSC4 domains critical for the interaction with U5 snRNP and the PRPF19 complex, as well as for TSSC4 function in tri-snRNP assembly. TSSC4 emerges as a specific chaperone that acts in U5 snRNP de novo biogenesis as well as post-splicing recycling. The correct assembly and recycling of the multicomponent spliceosome remains largely elusive. Here, the authors show that a previously uncharacterized protein TSSC4 associates with de novo formed spliceosomal U5 snRNP as well as with a post-splicing U5-PRPF19 particle, and that TSSC4 is important for assembly of the splicing competent tri-snRNP.
Permanent Link: http://hdl.handle.net/11104/0321024