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The sensitivity of multiple ecotoxicological assays for evaluating Microcystis aeruginosa cellular algal organic matter and contribution of cyanotoxins to the toxicity
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SYSNO ASEP 0542833 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The sensitivity of multiple ecotoxicological assays for evaluating Microcystis aeruginosa cellular algal organic matter and contribution of cyanotoxins to the toxicity Author(s) Šrédlová, Kamila (MBU-M) ORCID
Šilhavecká, Simona (MBU-M)
Linhartová, Lucie (MBU-M) RID, ORCID
Semerád, Jaroslav (MBU-M) ORCID, RID
Michalíková, Klára (MBU-M) ORCID
Pivokonský, Martin (UH-J) SAI, ORCID, RID
Cajthaml, Tomáš (MBU-M) RID, ORCIDSource Title Toxicon. - : Elsevier - ISSN 0041-0101
Roč. 195, MAY 2021 (2021), s. 69-77Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords Harmful algal blooms ; Microcystis aeruginosa ; Cellular algal organic matter ; Microcystin ; Rainbow trout cell lines ; Cytotoxicity OECD category Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management Subject RIV - cooperation Institute of Hydrodynamics - Water Pollution ; Quality R&D Projects GA18-14445S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 ; UH-J - RVO:67985874 UT WOS 000637220900010 EID SCOPUS 85102801768 DOI 10.1016/j.toxicon.2021.03.003 Annotation Secondary metabolites of cyanobacteria and algae released during algal blooms often exhibit toxic effects, but only a small number of the metabolites are the subject of routine analytical screenings. Alternatively, ecotoxicological assays offer a better representation of the overall negative effects. The aim of this work was to compare multiple assays in their sensitivity towards cellular algal organic matter (COM) of the toxin-producing cyanobacterium Microcystis aeruginosa. Multiple endpoints were investigated: mortality, growth inhibition, bioluminescence inhibition, genotoxicity, endocrine-disrupting effects, oxidative stress, and the induction of ethoxyresorufin-O-deethylase (EROD). Three rainbow trout (Oncorhynchus mykiss) cell lines as well as representatives of bacteria, yeasts, algae, vascular plants, and crustaceans were employed, and the results were expressed per mg of dissolved organic carbon (DOC) in the COM. M. aeruginosa COM was toxic to the RTgill-W1, RTG-2, and RTL-W1 cell lines (EC50 values ranging from 0.48 +/- 0.02 to 1.9 +/- 0.1 mg(DOC)/L), to the crustacean Thamnocephalus platyurus (LC50 = 20 +/- 1 mg(DOC)/L), and to Lepidium sativum (IC50 = 241 +/- 13 mg(DOC)/L). In contrast, no effect was observed for bacteria and yeasts, and the growth of the alga Desmodesmus subspicatus was even stimulated. No genotoxicity, endocrine-disrupting effects or increase in oxidative stress or EROD activity was detected. The content of six microcystins (MC-LR, MC-RR, MC-YR, MC-LY, MC-LW, and MC-LF), anatoxin-a, cylindrospermopsin, and nodularin in the M. aeruginosa COM was determined by liquid chromatography-tandem mass spectrometry. An artificially prepared mixture of the detected cyanotoxins in the corresponding concentrations did not induce response in the O. mykiss cell lines and T. platyurus, suggesting that other cyanobacterial metabolites are responsible for the toxicity of M. aeruginosa. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0041010121000763
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