The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts

Filová, Elena; Blanquer, Andreu; Knitlová, Jarmila; Plencner, Martin; Jenčová, V.; Kopřivová, B.; Lisnenko, M.; Kuželová Košťáková, E.; Procházková, R.; Bačáková, Lucie

doi:https://dx.doi.org/10.3390/nano11040995

Number of the records: 1

The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts

1.

SYSNO ASEP	0542427
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
Author(s)	Filová, Elena (FGU-C)_{RID, ORCID} Blanquer, Andreu (FGU-C)_{RID, ORCID} Knitlová, Jarmila (FGU-C)_{RID, ORCID} Plencner, Martin (FGU-C)_{RID, ORCID} Jenčová, V. (CZ) Kopřivová, B. (CZ) Lisnenko, M. (CZ) Kuželová Košťáková, E. (CZ) Procházková, R. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Article number	995
Source Title	Nanomaterials. - : MDPI Roč. 11, č. 4 (2021)
Number of pages	20 s.
Language	eng - English
Country	CH - Switzerland
Keywords	controlled release ; platelet lysate ; PVA nanofibers ; endothelial cells ; keratinocytes ; fibroblasts ; cell differentiation
Subject RIV	FB - Endocrinology, Diabetology, Metabolism, Nutrition
OECD category	Endocrinology and metabolism (including diabetes, hormones)
R&D Projects	NV18-01-00332 GA MZd - Ministry of Health (MZ)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000643386100001
EID SCOPUS	85104032421
DOI	10.3390/nano11040995
Annotation	Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% w/w). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA-PL nanomats. The bioactivity of the PVA-PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA-PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA-PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA-PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 mu m-sized pores. These results indicated the favorable effects of the PVA-PL nanomats on the three cell types involved in the wound healing process, and established PVA-PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2079-4991/11/4/995

Number of the records: 1