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Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions
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SYSNO ASEP 0541740 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Acetophenyl‐thienyl‐aniline‐Linked Nucleotide for Construction of Solvatochromic Fluorescence Light‐Up DNA Probes Sensing Protein‐DNA Interactions Author(s) Güixens-Gallardo, Pedro (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDSource Title Chemistry - A European Journal. - : Wiley - ISSN 0947-6539
Roč. 27, č. 24 (2021), s. 7090-7093Number of pages 4 s. Language eng - English Country DE - Germany Keywords fluorescent labelling ; DNA polymerases ; nucleotides ; protein-DNA interactions ; solvatochromism OECD category Biochemistry and molecular biology R&D Projects GA17-14791S GA ČR - Czech Science Foundation (CSF) EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000637991400001 EID SCOPUS 85104059102 DOI 10.1002/chem.202100575 Annotation The synthesis of 2′‐deoxycytidine and its 5′‐O‐triphosphate bearing solvatochromic acetophenyl‐thienyl‐aniline fluorophore was developed using the Sonogashira cross‐coupling reaction as the key step. The triphosphate was used for polymerase synthesis of labelled DNA. The labelled nucleotide or DNA exerted weak red fluorescence when excited at 405 nm, but a significant colour change (to yellow or green) and light‐up (up to 20 times) was observed when the DNA probes interacted with proteins or lipids. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2022 Electronic address https://doi.org/10.1002/chem.202100575
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