Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture

Dvořáková, Jana; Trousil, Jiří; Podhorská, Bohumila; Mikšovská, Zuzana; Janoušková, Olga; Proks, Vladimír

doi:https://dx.doi.org/10.1021/acs.biomac.0c01641

Number of the records: 1

Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture

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SYSNO ASEP	0541734
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture
Author(s)	Dvořáková, Jana (UMCH-V)_{RID, ORCID} Trousil, Jiří (UMCH-V)_{RID, ORCID} Podhorská, Bohumila (UMCH-V)_ORCID Mikšovská, Zuzana (UMCH-V) Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Proks, Vladimír (UMCH-V)_{RID, ORCID}
Source Title	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 22, č. 4 (2021), s. 1417-1431
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	poly(α-amino acid) ; enzymatic cross-linking ; enzymatic degradation
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
R&D Projects	GA18-03224S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000640310700008
EID SCOPUS	85103370049
DOI	https://doi.org/10.1021/acs.biomac.0c01641
Annotation	Injectable hydrogel scaffolds combined with stem cell therapy represent a promising approach for minimally invasive surgical tissue repair. In this study, we developed and characterized a fully synthetic, biodegradable poly(N5-(2-hydroxyethyl)-l-glutamine)-based injectable hydrogel modified with integrin-binding arginine–glycine–aspartic acid (RGD) peptide (PHEG-Tyr-RGD). The biodegradable hydroxyphenyl polymer precursor derivative of PHEG-Tyr was enzymatically cross-linked to obtain injectable hydrogels with different physicochemical properties. The gelation time, gel yield, swelling behavior, and storage modulus of the PHEG-Tyr hydrogels were tuned by varying the concentrations of the PHEG-Tyr precursors and horseradish peroxidase as well as the nH2O2/nTyr ratio. The mechanical properties and gelation time of the PHEG-Tyr hydrogel were optimized for the encapsulation of rat mesenchymal stem cells (rMSCs). We focused on the 2D and 3D spreading and viability of rMSCs within the PHEG-Tyr-RGD hydrogels with different physicochemical microenvironments in vitro. Encapsulation of rMSCs shows long-term survival and exhibits cell–matrix and cell–cell interactions reflective of both the RGD concentration and hydrogel stiffness. The presented biomaterial represents a suitable biological microenvironment to guide 3D spreading and may act as a promising 3D artificial extracellular matrix for stem cell therapy.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://pubs.acs.org/doi/10.1021/acs.biomac.0c01641

Number of the records: 1