Number of the records: 1
Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
- 1.
SYSNO ASEP 0541546 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats Author(s) Mrózková, Petra (FGU-C) RID, ORCID
Špicarová, Diana (FGU-C) RID, ORCID
Paleček, Jiří (FGU-C) RID, ORCIDArticle number 991 Source Title International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 22, č. 3 (2021)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords PAR2 ; TRPV1 ; synaptic transmission ; superficial dorsal horn ; spinal cord ; nociception ; peripheral inflammation ; thermal hyperalgesia ; inflammatory pain Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects GA18-09853S GA ČR - Czech Science Foundation (CSF) GA20-19136S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000615302300001 EID SCOPUS 85099703025 DOI https://doi.org/10.3390/ijms22030991 Annotation The mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/3/991
Number of the records: 1