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3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
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SYSNO ASEP 0541406 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice Author(s) Děd, Lukáš (BTO-N) RID
Hwang, J.Y. (US)
Miki, K. (US)
Shi, H. F. (US)
Chung, J.-J. (US)Number of authors 5 Article number e62043 Source Title eLife. - : eLife - ISSN 2050-084X
Roč. 9, OCT 20 2020 (2020)Number of pages 29 s. Language eng - English Country GB - United Kingdom Keywords Sperm Capacitation ; Acrosome Reaction ; Spermatozoa Subject RIV EB - Genetics ; Molecular Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000595597500001 EID SCOPUS 85095704918 DOI 10.7554/eLife.62043 Annotation Out of millions of ejaculated sperm, a few reach the fertilization site in mammals. Flagellar Ca2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection. Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades. CatSper1 processing correlates with protein tyrosine phosphorylation (pY) development in sperm cells capacitated in vitro and in vivo. Using 3D in situ molecular imaging and ANN-based automatic detection of sperm distributed along the cleared female tract, we demonstrate that spermatozoa past the utero-tubal junction possess the intact CatSper1 signals. Together, we reveal that fertilizing mouse spermatozoa in situ are characterized by intact CatSper channel, lack of pY, and reacted acrosomes. These findings provide molecular insight into sperm selection for successful fertilization in the female reproductive tract. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2021 Electronic address https://elifesciences.org/articles/62043
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