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Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS
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SYSNO ASEP 0541327 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS Author(s) Bosakova, T. (CZ)
Tockstein, A. (CZ)
Šebková, Nataša (BTO-N)
Čabala, R. (CZ)
Komrsková, Kateřina (BTO-N) ORCIDNumber of authors 5 Article number 124 Source Title Catalysts. - : MDPI
Roč. 10, č. 1 (2020)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords 17 alpha-ethynylestradiol ; EE2 ; sperm capacitation ; kinetics Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA18-11275S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000516825000124 EID SCOPUS 85078474738 DOI 10.3390/catal10010124 Annotation 17 alpha-Ethynylestradiol (EE2), a synthetic estrogen used in contraceptive pills, is resistant to hepatic degradation and is excreted in the urine. It is chemically stable and has a negative impact on the endocrine system. The aim of this work was to mathematically describe the possible interaction of EE2 (200, 20, and 2 mu g/L) with sperm estrogen receptors during sperm maturation, which is called capacitation. The concentrations of the unbound EE2 remaining in capacitating medium during 180 min of sperm capacitation were determined at 30 min intervals by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and the data obtained (relative concentrations B-t) were subjected to kinetic analysis. The suggested kinetic schema was described by the system of differential equations with the optimization of rate constants used to calculate the theoretical B-t values. Optimal parameters (overall rate constants K-1-K-5 and molar ratio n) were determined by searching the minimum of absolute values of the difference between theoretical and experimental B-t values. These values were used for the design of the theoretical B(t) curves which fit to experimental points. The proposed kinetic model assumes the formation of an unstable adduct between EE2 and the receptor in cytoplasm, which acts as an autocatalytic agent and gradually decomposes. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2021 Electronic address https://www.mdpi.com/2073-4344/10/1/124
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