0541022 - BC 2021 RIV CZ eng A - Abstract
Vancová, Marie - Dolejší, M. - Vaněček, Jiří - Skoupý, Radim - Krzyžánek, Vladislav - Nebesářová, Jana
POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope.
CSMS conference Microscopy 2020, online meeting 5th-6th 2020. Praha: Československá mikroskopická společnost, z.s., 2020. s. 57-57.
[CSMS conference Microscopy 2020, online meeting 6th-7th 2020. 06.10.2021-07.10.2021, Lednice]
R&D Projects: GA MŠMT(CZ) 8J19AT009; GA ČR(CZ) GA17-21244S
Institutional support: RVO:60077344 ; RVO:68081731
Keywords : Nanomechanical * Lyme disease * extracellular matrix
OECD category: Microbiology; Electrical and electronic engineering (UPT-D)
Result website:
http://microscopy.cz/conferencecsms/csms-sbornik.pdf

As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochaetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly DbpA/B, can strongly enhance motility of spirochetes in the extracellular matrix of the host. Using atomic force microscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, in order to switch between different motility states to enhance its dissemination
Permanent Link: http://hdl.handle.net/11104/0318613