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The Lipid Composition of Euglena gracilis Middle Plastid Membrane Resembles That of Primary Plastid Envelopes
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SYSNO ASEP 0540689 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Lipid Composition of Euglena gracilis Middle Plastid Membrane Resembles That of Primary Plastid Envelopes Author(s) Tomečková, A. (CZ)
Tomčala, Aleš (BC-A) RID
Oborník, Miroslav (BC-A) RID, ORCID
Hampl, V. (CZ)Number of authors 4 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 184, č. 4 (2020), s. 2052-2063Number of pages 12 s. Publication form Print - P Language eng - English Country US - United States Keywords trap mass-spectrometry ; chloroplast genome ; complex chloroplasts ; fatty-acid ; protein ; biosynthesis ; evolution ; phospholipids ; synthase ; light Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 000601303200038 EID SCOPUS 85097506177 DOI 10.1104/pp.20.00505 Annotation The lipid composition of E. gracilis plastid envelopes suggests that the two innermost membranes are rich in glycosyldiacylglycerols, indicating their similarity to primary plastid membranes.Euglena gracilis is a photosynthetic flagellate possessing chlorophyte-derived secondary plastids that are enclosed by only three enveloping membranes, unlike most secondary plastids, which are surrounded by four membranes. It has generally been assumed that the two innermost E. gracilis plastid envelopes originated from the primary plastid, while the outermost is of eukaryotic origin. It was suggested that nucleus-encoded plastid proteins pass through the middle and innermost plastid envelopes of E. gracilis by machinery homologous to the translocons of outer and inner chloroplast membranes, respectively. Although recent genomic, transcriptomic, and proteomic data proved the presence of a reduced form of the translocon of inner membrane, they failed to identify any outer-membrane translocon homologs, which raised the question of the origin of E. gracilis's middle plastid envelope. Here, we compared the lipid composition of whole cells of the pigmented E. gracilis strain Z and two bleached mutants that lack detectable plastid structures, W10BSmL and WgmZOflL. We determined the lipid composition of E. gracilis strain Z mitochondria and plastids, and of plastid subfractions (thylakoids and envelopes), using HPLC high-resolution tandem mass spectrometry, thin-layer chromatography, and gas chromatography-flame ionization detection analytical techniques. Phosphoglycerolipids are the main structural lipids in mitochondria, while glycosyldiacylglycerols are the major structural lipids of plastids and also predominate in extracts of whole mixotrophic cells. Glycosyldiacylglycerols were detected in both bleached mutants, indicating that mutant cells retain some plastid remnants. Additionally, we discuss the origin of the E. gracilis middle plastid envelope based on the lipid composition of envelope fraction. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://academic.oup.com/plphys/article/184/4/2052/6119037
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