ADAMTS Proteases: Methods and Protocols

0540393 - ÚEM 2021 US eng M - Monography Chapter
Souter, L. - Kwok, Jessica
Visualization of Perineuronal Nets in Central Nervous System Tissue Sections.
ADAMTS Proteases: Methods and Protocols. HUMANA PRESS INC, 2020 - (Apte, S.), s. 251-260. ISBN 978-1-4939-9698-8; 978-1-4939-9697-1
R&D Projects: GA MŠMT(CZ) EF15_003/0000419
Institutional support: RVO:68378041
Keywords : perineuronal nets * wisteria floribunda agglutinin * extracellular-matrix
OECD category: Neurosciences (including psychophysiology

The perineuronal net (PNN) is a specialized extracellular matrix structure that surrounds subpopulations of neurons in the central nervous system (CNS). The appearance of PNNs on the cell surface marks the closure of the critical period during development and has been observed to reduce synaptic plasticity. Perineuronal nets comprise hyaluronan, chondroitin sulfate proteoglycans (CSPGs), link proteins, tenascin-R, and other components, some of which are substrates for a disintegrin-like and metalloprotease domain with thrombospondin type 1 motifs (ADAMTS) proteases. There is a high heterogeneity of PNNs in the CNS. Depending on which part of the CNS is studied, the PNNs may be observed surrounding the soma, or both the soma and proximal dendrites. The most robust marker for PNN is a lectin called Wisteria floribunda agglutinin. Here, we describe a method for preparing tissue for visualization of PNNs in CNS.
Permanent Link: http://hdl.handle.net/11104/0318015