Challenge-comet assay, a functional and genomic biomarker for precision risk assessment and disease prevention among exposed workers

0538525 - ÚEM 2021 RIV US eng J - Journal Article
Xu, J. - Šrám, Radim - Cebulska-Wasilewska, A. - Miloradov, M.D. - Sardas, S. - Au, W.W.
Challenge-comet assay, a functional and genomic biomarker for precision risk assessment and disease prevention among exposed workers.
Toxicology and Applied Pharmacology. Roč. 397, jun (2020), č. článku 115011. ISSN 0041-008X. E-ISSN 1096-0333
R&D Projects: GA MŠMT(CZ) EF16_019/0000798
Institutional support: RVO:68378041
Keywords : DNA damage and repair response * challenge assay * Comet/hComet assay
OECD category: Public and environmental health
Impact factor: 4.219, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/abs/pii/S0041008X20301356?via%3Dihub

Advancements in genomic technologies have ushered application of innovative changes into biomedical sciences and clinical medicine. Consequently, these changes have created enormous opportunities to implement precision population/occupational disease prevention and target-specific disease intervention (or personalized medicine). To capture the opportunities, however, it is necessary is to develop novel, especially genomic-based, biomarkers which can provide precise and individualized health risk assessment. In this review, development of the Challenge-comet assay is used as an example to demonstrate how assays need to be validated for its sensitivity, specificity, and functional and quantitative features, and how assays can be used to provide individualized health risk assessment for precision prevention and intervention. Other examples of genomic-based novel biomarkers will also be discussed. Furthermore, no biomarkers can be used alone therefore their integrated usage with other biomarkers and with personal data bases will be discussed.
Permanent Link: http://hdl.handle.net/11104/0316318