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Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
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SYSNO ASEP 0537933 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies Author(s) Němčáková, Ivana (FGU-C) RID, ORCID
Blahová, L. (CZ)
Ryšánek, P. (CZ)
Blanquer, Andreu (FGU-C) RID, ORCID
Bačáková, Lucie (FGU-C) RID, ORCID
Zajíčková, L. (CZ)Article number 9467 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 24 (2020)Number of pages 24 s. Language eng - English Country CH - Switzerland Keywords amine plasma polymer ; bioactive coating ; polycaprolactone nanofibers ; substrate morphology ; cell adhesion ; cell adhesion Subject RIV BK - Fluid Dynamics OECD category Fluids and plasma physics (including surface physics) R&D Projects GA18-12774S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzechNanoLab - 90110 - Vysoké učení technické v Brně Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000602830000001 EID SCOPUS 85097659712 DOI 10.3390/ijms21249467 Annotation Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://www.mdpi.com/1422-0067/21/24/9467
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