Chronic n-3 fatty acid intake enhances insulin response to oral glucose and elevates GLP-1 in high-fat diet-fed obese mice

Pavlišová, Jana; Horáková, Olga; Kalendová, Veronika; Burešová, Jana; Bardová, Kristina; Holendová, Blanka; Plecitá-Hlavatá, Lydie; Vacková, Šárka; Windrichová, J.; Topolčan, O.; Kopecký, Jan; Rossmeisl, Martin

doi:https://dx.doi.org/10.1039/d0fo01942a

Number of the records: 1

Chronic n-3 fatty acid intake enhances insulin response to oral glucose and elevates GLP-1 in high-fat diet-fed obese mice

1.

SYSNO ASEP	0537893
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Chronic n-3 fatty acid intake enhances insulin response to oral glucose and elevates GLP-1 in high-fat diet-fed obese mice
Author(s)	Pavlišová, Jana (FGU-C) Horáková, Olga (FGU-C)_{RID, ORCID} Kalendová, Veronika (FGU-C)_{RID, ORCID} Burešová, Jana (FGU-C)_{ORCID, RID} Bardová, Kristina (FGU-C)_{RID, ORCID, SAI} Holendová, Blanka (FGU-C)_{RID, ORCID, SAI} Plecitá-Hlavatá, Lydie (FGU-C)_{RID, ORCID} Vacková, Šárka (FGU-C) Windrichová, J. (CZ) Topolčan, O. (CZ) Kopecký, Jan (FGU-C)_{RID, ORCID} Rossmeisl, Martin (FGU-C)_{RID, ORCID}
Source Title	Food & Function. - : Royal Society of Chemistry - ISSN 2042-6496 Roč. 11, č. 11 (2020), s. 9764-9775
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	obesity ; omega-3 fatty acids ; oGTT ; incretin hormones ; GSIS ; glucagon-like peptide-1 ; C57BL/6N mice
Subject RIV	FB - Endocrinology, Diabetology, Metabolism, Nutrition
OECD category	Endocrinology and metabolism (including diabetes, hormones)
R&D Projects	GA17-11027S GA ČR - Czech Science Foundation (CSF)
	GA16-08124S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	FGU-C - RVO:67985823
UT WOS	000592490800032
EID SCOPUS	85096508581
DOI	10.1039/d0fo01942a
Annotation	n-3 polyunsaturated fatty acids (PUFA) can exert beneficial effects on glucose homeostasis, especially in obese rodents. Gut incretin hormones regulate glucose and lipid homeostasis, but their involvement in the above effects is not entirely clear. This study aims to assess the effects of chronic n-3 PUFA administration on the insulin and incretin responses in C57BL/6N obese male mice subjected to oral glucose tolerance test (oGTT) after 8 weeks of feeding a corn-oil-based high-fat diet (cHF). The weight gain and adiposity were partially reduced in mice fed cHF in which some of the corn oil was replaced with n-3 PUFA concentrate containing similar to 60% DHA and EPA in a 3 : 1 ratio. In addition, these mice had improved glucose tolerance, which was consistent with an increased insulin response to oral glucose and plasma glucagon-like peptide-1 (GLP-1) levels. While the stimulatory effects of n-3 PUFA on GLP-1 levels could not be attributed to changes in intestinal or plasma dipeptidyl peptidase-4 activity, their beneficial effects on glucose tolerance were abolished when mice were pretreated with the GLP-1 receptor antagonist exendin 9-39. Moreover, chronic n-3 PUFA intake prevented the detrimental effects of cHF feeding on glucose-stimulated insulin secretion in the pancreatic islets. Collectively, our data suggest that n-3 PUFA may modulate postprandial glucose metabolism in obese mice through a GLP-1-based mechanism. The significance of these findings in terms of the effective DHA and EPA ratio of the n-3 PUFA concentrate as well as the effect of n-3 PUFA in humans requires further research.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2021
Electronic address	https://doi.org/10.1039/D0FO01942A

Number of the records: 1