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Directionality of light absorption and emission in representative fluorescent proteins
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SYSNO ASEP 0537175 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Directionality of light absorption and emission in representative fluorescent proteins Author(s) Myšková, Jitka (UOCHB-X) ORCID, RID
Rybakova, Olga (UOCHB-X) ORCID
Brynda, Jiří (UOCHB-X) RID, ORCID
Khoroshyy, Petro (UOCHB-X) ORCID, RID
Bondar, Alexey (UOCHB-X) ORCID, RID
Lazar, Josef (UOCHB-X) ORCID, RIDSource Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 117, č. 51 (2020), s. 32395-32401Number of pages 7 s. Language eng - English Country US - United States Keywords fluorescent protein ; transition dipole moment ; polarization microscopy Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000601315200031 EID SCOPUS 85098213266 DOI 10.1073/pnas.2017379117 Annotation Fluorescent proteins have been used extensively in many areas of life sciences. Many of their applications rely on their various biophysical properties, such as excitation and emission wavelengths, excited state lifetimes, or sensitivity to the molecular environment. One aspect of fluorescent proteins that has remained largely neglected is the directionality of their optical properties. In the present work, we describe our experimental determination of the directionality of light absorption and emission in several commonly used fluorescent proteins. Our findings improve our understanding of fundamental properties of fluorescent proteins, and expand the possibilities of development and applications of genetically encoded fluorescent probes. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2021 Electronic address https://doi.org/10.1073/pnas.2017379117
Number of the records: 1