Number of the records: 1  

Simultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium

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    SYSNO ASEP0536958
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleSimultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium
    Author(s) Kopejtka, Karel (MBU-M) ORCID, RID
    Tomasch, J. (DE)
    Zeng, Yonghui (MBU-M) ORCID
    Selyanin, Vadim (MBU-M) RID
    Dachev, Marko (MBU-M) ORCID
    Piwosz, Kasia (MBU-M) ORCID
    Tichý, Martin (MBU-M) RID
    Bína, David (BC-A) RID, ORCID
    Gardian, Zdenko (BC-A) RID
    Bunk, B. (DE)
    Brinkmann, H. (DE)
    Geffers, R. (DE)
    Sommaruga, R. (AT)
    Koblížek, Michal (MBU-M) RID, ORCID
    Article numbere01044-20
    Source TitlemSystems. - : American Society for Microbiology - ISSN 2379-5077
    Roč. 5, č. 6 (2020)
    Number of pages17 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsAerobic anoxygenic phototrophic bacteria ; Bacteriochlorophyll ; Gene expression ; Photosynthesis gene cluster ; Rhodopsin
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    Subject RIV - cooperationBiology Centre (since 2006) - Physiology
    R&D ProjectsGJ18-14095Y GA ČR - Czech Science Foundation (CSF)
    Method of publishingOpen access
    Institutional supportMBU-M - RVO:61388971 ; BC-A - RVO:60077344
    UT WOS000630974900017
    EID SCOPUS85098211480
    DOI10.1128/mSystems.01044-20
    AnnotationPhotoheterotrophic bacteria represent an important part of aquatic microbial communities. There exist two fundamentally different light-harvesting systems: bacteriochlorophyll-containing reaction centers or rhodopsins. Here, we report a photoheterotrophic Sphingomonas strain isolated from an oligotrophic lake, which contains complete sets of genes for both rhodopsin-based and bacteriochlorophyllbased phototrophy. Interestingly, the identified genes were not expressed when cultured in liquid organic media. Using reverse transcription quantitative PCR (RT-qPCR), RNA sequencing, and bacteriochlorophyll a quantification, we document that bacteriochlorophyll synthesis was repressed by high concentrations of glucose or galactose in the medium. Coactivation of photosynthesis genes together with genes for TonBdependent transporters suggests the utilization of light energy for nutrient import. The photosynthetic units were formed by ring-shaped light-harvesting complex 1 and reaction centers with bacteriochlorophyll a and spirilloxanthin as the main lightharvesting pigments. The identified rhodopsin gene belonged to the xanthorhodopsin family, but it lacks salinixanthin antenna. In contrast to bacteriochlorophyll, the expression of xanthorhodopsin remained minimal under all experimental conditions tested. Since the gene was found in the same operon as a histidine kinase, we propose that it might serve as a light sensor. Our results document that photoheterotrophic Sphingomonas bacteria use the energy of light under carbon-limited conditions, while under carbon-replete conditions, they cover all their metabolic needs through oxidative phosphorylation. IMPORTANCE Phototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2021
    Electronic addresshttps://msystems.asm.org/content/5/6/e01044-20
Number of the records: 1  

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