Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

0536183 - BFÚ 2021 RIV GB eng J - Journal Article
Špaček, Jan - Fojta, Miroslav
Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism.
Electrochimica acta. Roč. 364, Dec 20 2020 (2020), č. článku 137298. ISSN 0013-4686. E-ISSN 1873-3859
R&D Projects: GA ČR GBP206/12/G151; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : electrochemical reduction * nucleic-acids * expansion * electrocatalysis * voltammetry * electrodes * evolution * proteins * probes
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 6.901, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub

While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0313995