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Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD
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SYSNO ASEP 0535059 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD Author(s) Proctor, M. S. (GB)
Pazderník, Marek (MBU-M)
Jackson, P. J. (GB)
Pilný, Jan (MBU-M) ORCID
Martin, E. C. (GB)
Dickman, M. J. (GB)
Canniffe, D. P. (GB)
Johnson, M. P. (GB)
Hunter, C. N. (GB)
Sobotka, Roman (MBU-M) RID, ORCID
Hitchcock, A. (GB)Source Title Biochemical Journal. - : Portland Press - ISSN 0264-6021
Roč. 477, č. 20 (2020), s. 4021-4036Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords light-inducible polypeptides ; synechocystis sp pcc-6803 ; photosystem-ii ; beta-carotene ; biosynthetic-pathway ; bind chlorophyll ; zeaxanthin ; myxoxanthophyll Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GX19-29225X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000588274400004 EID SCOPUS 85094983874 DOI 10.1042/BCJ20200561 Annotation Chlorophyll synthase (ChlG) catalyses a terminal reaction in the chlorophyll biosynthesis pathway, attachment of phytol or geranylgeraniol to the C17 propionate of chlorophyllide. Cyanobacterial ChlG forms a stable complex with high light-inducible protein D (HliD), a small single-helix protein homologous to the third transmembrane helix of plant light-harvesting complexes (LHCs). The ChlG-HliD assembly binds chlorophyll, beta-carotene, zeaxanthin and myxoxanthophyll and associates with the YidC insertase, most likely to facilitate incorporation of chlorophyll into translated photosystem apoproteins. HliD independently coordinates chlorophyll and beta-carotene but the role of the xanthophylls, which appear to be exclusive to the core ChlG-HliD assembly, is unclear. Here we generated mutants of Synechocystis sp. PCC 6803 lacking specific combinations of carotenoids or HliD in a background with FLAG- or His-tagged ChlG. Immunoprecipitation experiments and analysis of isolated membranes demonstrate that the absence of zeaxanthin and myxoxanthophyll significantly weakens the interaction between HliD and ChlG. ChlG alone does not bind carotenoids and accumulation of the chlorophyllide substrate in the absence of xanthophylls indicates that activity/stability of the `naked' enzyme is perturbed. In contrast, the interaction of HliD with a second partner, the photosystem II assembly factor Ycf39, is preserved in the absence of xanthophylls. We propose that xanthophylls are required for the stable association of ChlG and HliD, acting as a ´molecular glue´ at the lateral transmembrane interface between these proteins, roles for zeaxanthin and myxoxanthophyll in ChlG-HliD complexation are discussed, as well as the possible presence of similar complexes between LHC-like proteins and chlorophyll biosynthesis enzymes in plants. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://portlandpress.com/biochemj/article-abstract/477/20/4021/226545/Xanthophyll-carotenoids-stabilise-the-association?redirectedFrom=fulltext
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