New multimodal stationary phases prepared by Ugi multicomponent approach

0534797 - ÚMCH 2021 RIV DE eng J - Journal Article
Sýkora, D. - Záruba, K. - Butnariu, M. - Tatar, A. - Pham, H. M. - Studenovský, Martin - Řezanka, P. - Král, V.
New multimodal stationary phases prepared by Ugi multicomponent approach.
Journal of Separation Science. Roč. 43, č. 22 (2020), s. 4178-4190. ISSN 1615-9306. E-ISSN 1615-9314
Institutional support: RVO:61389013
Keywords : hydrophilic interaction * hydrophobic interaction * ion-exchange interaction
OECD category: Polymer science
Impact factor: 3.645, year: 2020
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/10.1002/jssc.202000587

Eight different stationary phases based on two aminopropyl silicas of different brands suitable for multimodal chromatography applications have been prepared by a four‐component Ugi reaction. The intention was to synthesize stationary phases significantly differing in their properties hereby demonstrating flexibility of the Ugi synthetic protocol. Diverse functional groups including a nonpolar long aliphatic chain, phenyl moiety, cholic acid scaffold, phenylboronic and monosaccharide units, charged betaine, and arginine moieties were immobilized on a silica surface. The novel sorbents were extensively characterized by elemental analysis, Raman spectroscopy, and chromatography. Considering the anchored chemical structures covalently bonded to the silica surface, reversed‐phase, hydrophilic, and ion‐exchange separation modes were expected. The chromatographic evaluation was performed directed to map the potential of the individual columns specifically in the mentioned chromatographic modes. The Ugi synthetic protocol has proven to be a simple, feasible, and versatile tool for the synthesis of sorbents of variable properties. The newly prepared stationary phases differed considerably in hydrophobicity and ion‐exchange ability. A significant influence of the supporting aminopropyl silica on the final chromatographic behavior was observed. Finally, one practical example confirming applicability of the newly prepared sorbents was demonstrated in separation of cytarabine.
Permanent Link: http://hdl.handle.net/11104/0313251