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Genotoxicant exposure, activation of the aryl hydrocarbon receptor, and lipid peroxidation in cultured human alveolar type II A549 cells
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SYSNO ASEP 0532294 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genotoxicant exposure, activation of the aryl hydrocarbon receptor, and lipid peroxidation in cultured human alveolar type II A549 cells Author(s) Rössner ml., Pavel (UEM-P) RID, ORCID
Líbalová, Helena (UEM-P) RID
Vrbová, Kristýna (UEM-P)
Červená, Tereza (UEM-P) ORCID, RID
Rössnerová, Andrea (UEM-P) RID
Elzeinová, Fatima (UEM-P)
Milcová, Alena (UEM-P)
Nováková, Zuzana (UEM-P)
Topinka, Jan (UEM-P) RID, ORCIDArticle number 503173 Source Title Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
Roč. 853, SI (2020)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords aryl hydrocarbon receptor ; extractable organic matter ; lipid peroxidation Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA16-14631S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UEM-P - RVO:68378041 UT WOS 000540226200004 EID SCOPUS 85082849142 DOI https://doi.org/10.1016/j.mrgentox.2020.503173 Annotation The aryl hydrocarbon receptor (AhR) transcription factor is activated by polycyclic aromatic hydrocarbons (PAH) and other ligands. Activated AhR binds to dioxin responsive elements (DRE) and initiates transcription of target genes, including the gene encoding prostaglandin endoperoxide synthase 2 (PTGS-2), which is also activated by the transcription factor NF-kappa B. PTGS-2 catalyzes the conversion of arachidonic acid (AA) into prostaglandins, thromboxanes or isoprostanes. 15-F2t-Isoprostane (IsoP), regarded as a universal marker of lipid peroxidation, is also induced by PAH exposure. We investigated the processes associated with lipid peroxidation in human alveolar basal epithelial cells (A549) exposed for 4 h or 24 h to model PAH (benzo[a]pyrene, BaP, 3-nitrobenzanthrone, 3-NBA) and organic extracts from ambient air particulate matter (EOM), collected in two seasons in a polluted locality. Both EOM induced the expression of CYP1A1 and CYP1B1, 24 h treatment significantly reduced PTGS-2 expression. IsoP levels decreased after both exposure periods, while the concentration of AA was not affected. The effects induced by BaP were similar to EOM except for increased IsoP levels after 4 h exposure and elevated AA concentration after 24 h treatment. In contrast, 3-NBA treatment did not induce CYP expression, had a weak effect on PTGS-2 expression, and, similar to BaP, induced IsoP levels after 4 h exposure and AA levels after 24 h treatment. All tested compounds induced the activity of NF-kappa B after the longer exposure period. In summary, our data suggest that EOM, and partly BaP, reduce lipid peroxidation by a mechanism that involves AhR-dependent inhibition of PTGS-2 expression. The effect of 3-NBA on IsoP levels is probably mediated by a different mechanism independent of AhR activation. Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2021 Electronic address https://www.sciencedirect.com/science/article/abs/pii/S1383571820300437?via%3Dihub
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