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Cell Distribution within Yeast Colonies and Colony Biofilms: How Structure Develops
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SYSNO ASEP 0532060 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cell Distribution within Yeast Colonies and Colony Biofilms: How Structure Develops Author(s) Plocek, V. (CZ)
Váchová, Libuše (MBU-M) RID, ORCID
Šťovíček, V. (CZ)
Palková, Z. (CZ)Article number 3873 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 11 (2020)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords yeast multicellular structures ; colonies and biofilms ; structure development Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA19-11384S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000543400300135 EID SCOPUS 85085908613 DOI 10.3390/ijms21113873 Annotation Multicellular structures formed by yeasts and other microbes are valuable models for investigating the processes of cell-cell interaction and pattern formation, as well as cell signaling and differentiation. These processes are essential for the organization and development of diverse microbial communities that are important in everyday life. Two major types of multicellular structures are formed by yeast Saccharomyces cerevisiae on semisolid agar. These are colonies formed by laboratory or domesticated strains and structured colony biofilms formed by wild strains. These structures differ in spatiotemporal organization and cellular differentiation. Using state-of-the-art microscopy and mutant analysis, we investigated the distribution of cells within colonies and colony biofilms and the involvement of specific processes therein. We show that prominent differences between colony and biofilm structure are determined during early stages of development and are associated with the different distribution of growing cells. Two distinct cell distribution patterns were identified-the zebra-type and the leopard-type, which are genetically determined. The role of Flo11p in cell adhesion and extracellular matrix production is essential for leopard-type distribution, because FLO11 deletion triggers the switch to zebra-type cell distribution. However, both types of cell organization are independent of cell budding polarity and cell separation as determined using respective mutants. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.mdpi.com/1422-0067/21/11/3873
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