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Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors
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SYSNO ASEP 0531903 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors Author(s) Pobořilová, Zuzana (UEB-Q) ORCID, RID
Plchová, Helena (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Gunter, C. J. (ZA)
Hitzeroth, I. I. (ZA)
Rybicki, E. P. (ZA)
Moravec, Tomáš (UEB-Q) RID, ORCIDNumber of authors 7 Source Title Plant Cell Reports. - : Springer - ISSN 0721-7714
Roč. 39, č. 9 (2020), s. 1115-1127Number of pages 13 s. Language eng - English Country DE - Germany Keywords DsRed ; gfp ; Plant cell pack ; Plant expression vector ; Tobacco BY-2 cells ; Transient co-expression Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA15-10768S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TG03010009 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000528435000001 EID SCOPUS 85084142930 DOI 10.1007/s00299-020-02544-w Annotation Key message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs.
Abstract: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.1007/s00299-020-02544-w
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