Number of the records: 1
Salt-washed graphene oxide and its cytotoxicity
- 1.
SYSNO ASEP 0531634 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Salt-washed graphene oxide and its cytotoxicity Author(s) Mrózek, Ondřej (UACH-T) RID, ORCID, SAI
Melounková, L. (CZ)
Smržová, Darina (UACH-T) ORCID, SAI, RID
Machálková, Aneta (UACH-T) SAI, ORCID, RID
Vinklárek, J. (CZ)
Němečková, Zuzana (UACH-T) SAI, RID, ORCID
Komárková, Bára (UACH-T) RID, SAI, ORCID
Ecorchard, Petra (UACH-T) SAI, RID, ORCIDNumber of authors 8 Article number 123114 Source Title Journal of Hazardous Materials. - : Elsevier - ISSN 0304-3894
Roč. 398, NOV (2020)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Cytotoxicity ; Graphene oxide ; HDF ; MRC-5 cell cultures ; Purification ; Salt-washing Subject RIV CA - Inorganic Chemistry OECD category Inorganic and nuclear chemistry R&D Projects GA17-08273S GA ČR - Czech Science Foundation (CSF) LM2018124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access with time embargo (11.06.2022) Institutional support UACH-T - RVO:61388980 UT WOS 000568851700002 EID SCOPUS 85086382026 DOI 10.1016/j.jhazmat.2020.123114 Annotation The carbon nanomaterials and congeners, e.g., graphene or graphene oxide (GO), dispose of numerous unique properties, which are not necessarily intrinsic but might be related to a content of impurities. The oxidation step of GO synthesis introduces a considerable amount of metallic species. Therefore, large-scale purification is an actual scientific challenge. Here we describe new purification technique (salt‑washing), which is based on three consecutive steps: (a) aggregation of GO sheets with NaCl (b) washing of the aggregates and (c) removing of the salt to afford purified GO (swGO). The considerably improved purity of swGO was demonstrated by ICP and EPR spectroscopy. The microscopic methods (TEM with SEAD, AFM) proved that the salt-washing does not affect the morphology or concentration of defects, showing the aggregation of GO with NaCl is fully reversible. The eligibility of swGO for biomedical applications was tested using fibroblastic cell cultures. The determined IC50 values clearly show a strong correlation between the purity of samples and cytotoxicity. Although the purification decreases cytotoxicity of GO, the IC50 values are still low proving that cytotoxic effect is not only impurities-related but also an intrinsic property. These findings may represent a serious limitation for usage of GO in biomedical applications. Workplace Institute of Inorganic Chemistry Contact Jana Kroneislová, krone@iic.cas.cz, Tel.: 311 236 931 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0310259
Number of the records: 1