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gamma-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis
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SYSNO ASEP 0524624 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title gamma-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis Author(s) Kallai, B.M. (HU)
Kourová, Hana (MBU-M)
Chumová, Jana (MBU-M)
Papdi, C. (GB)
Trögelová, Lucie (MBU-M) ORCID
Kofroňová, Olga (MBU-M) RID, ORCID
Hozák, Pavel (UMG-J) RID, ORCID
Filimonenko, Vlada (UMG-J) RID, ORCID
Mészáros, T. (HU)
Magyar, Z. (HU)
Börge, L. (GB)
Binarová, Pavla (MBU-M) RID, ORCIDSource Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 71, č. 4 (2020), s. 1265-1277Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis ; endoreduplication ; E2F transcription factors Subject RIV EE - Microbiology, Virology OECD category Cell biology Subject RIV - cooperation Institute of Molecular Genetics R&D Projects GA15-11657S GA ČR - Czech Science Foundation (CSF) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 UT WOS 000518530400009 EID SCOPUS 85080840986 DOI 10.1093/jxb/erz498 Annotation gamma-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it also functions in nuclear processes and in cell division control independently of its canonical role. We found that in Arabidopsis thaliana, gamma-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of gamma-tubulin with the E2Fs is not reduced in the presence of their dimerization partners (DPs) and, in agreement, we found that gamma-tubulin interaction with E2Fs does not require the dimerization domain. gamma-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F-dependent manner, probably in complex with the E2F-DP heterodimer. The up-regulation of E2F target genes PCNA, ORC2, CDKB1,1, and CCS52A under gamma-tubulin silencing suggests a repressive function for gamma-tubulin at G(1)/S and G(2)/M transitions, and the endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of gamma-tubulin RNAi plants. Altogether, our data show ternary interaction of gamma-tubulin with the E2F-DP heterodimer and suggest a repressive role for gamma-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://academic.oup.com/jxb/article/71/4/1265/5613973
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