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Nanosecond Pulsed Electric Field Lab-on-Chip Integrated in Super-Resolution Microscope for Cytoskeleton Imaging
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SYSNO ASEP 0524260 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanosecond Pulsed Electric Field Lab-on-Chip Integrated in Super-Resolution Microscope for Cytoskeleton Imaging Author(s) Havelka, Daniel (URE-Y) RID
Chafai, Djamel Eddine (URE-Y)
Krivosudský, Ondrej (URE-Y)
Klebanovych, Anastasiya (UMG-J)
Vostárek, František (FGU-C) RID, ORCID, SAI
Kubínová, Lucie (FGU-C) RID, ORCID
Dráber, Pavel (UMG-J) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 8 Article number 1900669 Source Title Advanced Materials Technologies - ISSN 2365-709X
Roč. 5, č. 3 (2020)Number of pages 9 s. Publication form Print - P Language eng - English Country US - United States Keywords chips ; electromagnetics ; microtubules ; nsPEF Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Biophysics Subject RIV - cooperation Institute of Molecular Genetics - Biophysics
Institute of Physiology - Cell BiologyR&D Projects GA18-23597S GA ČR - Czech Science Foundation (CSF) GA17-11898S GA ČR - Czech Science Foundation (CSF) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-20716S GA ČR - Czech Science Foundation (CSF) Research Infrastructure Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Limited access Institutional support URE-Y - RVO:67985882 ; FGU-C - RVO:67985823 ; UMG-J - RVO:68378050 UT WOS 000492740600001 EID SCOPUS 85074616711 DOI 10.1002/admt.201900669 Annotation Nanosecond pulsed electric field offers novel opportunities in bionanotechnology and biomedicine enabling ultrafast physical control of membrane, and protein-based processes for the development of novel bionanomaterials and biomedical theranostic methods. However, the mechanisms of nanosecond pulsed electric field action at the nano- and molecular scale are not fully understood due to lack of appropriate research tools. In order to overcome this challenge, a technological platform for the exploration of these mechanisms in live biological samples is provided here. This paper describes step by step the proposed chip platform, including the design, fabrication, installation, and testing of the chip. The developed chip is capable of delivering hundreds of volts of nanosecond electric pulses compared to conventional chips using few volts. Moreover, the chip is fully integrated into a super-resolution microscope. Later on, the chip function is demonstrated by affecting microtubule architecture in living cells. Therefore, the chip-based technological advancement enables the assessment of pulsed electric field effects on bionanostructures and observing their effects in real-time. The results contribute to the chip-based high-frequency bioelectronics technology for modulating the function of biological matter at the nanoscale level Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2021 Electronic address https://doi.org/10.1002/admt.201900669
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