Antibacterial activity of chitosan/collagen membranes containing red propolis extract

0524090 - ÚMCH 2021 RIV DE eng J - Journal Article
Loureiro, K. C. - Barbosa, T. C. - Nery, M. - Chaud, M. V. - da Silva, C. F. - Andrade, L. N. - Corrêa, C. B. - Jäger, Alessandro - Padilha, F. F. - Cardoso, J. C. - Souto, E. - Severino, P.
Antibacterial activity of chitosan/collagen membranes containing red propolis extract.
Pharmazie. Roč. 75, 2-3 (2020), s. 75-81. ISSN 0031-7144. E-ISSN 0031-7144
Institutional support: RVO:61389013
Keywords : mucoadhesive polymeric membranes * red propolis extract * antibacterial
OECD category: Polymer science
Impact factor: 1.267, year: 2020
Method of publishing: Limited access
https://www.ingentaconnect.com/contentone/govi/pharmaz/2020/00000075/f0020002/art00005

In this study we developed a mucoadhesive polymeric membrane wound dressing incorporating red propolis extract (HERP). Membranes were made using a casting method employing collagen, chitosan, polyethylene glycol (15, 20, and 30v%), and hydroethanolic extract of EtOH-H2O 70v% – 30v% (v/v) of HERP (0.5, 1.0, and 1.5%). Membranes were extensively characterized to assess the thickness, pH, morphology using Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), mechanical properties, swelling, in vitro mucoadhesion, cytotoxicity, and minimum inhibitory concentration (MIC). Assessment of the thickness and mechanical properties of the membranes containing HERP revealed that the most significant thickness obtained was 40.7 μm, thermal analysis suggests suggesting the hydrogen bonds between hydroxyl groups of isoflavones and the free amine present in the region of chitosan. Cell viability decreased as the amount of HERP increased. Finally, the MICs were 7.8 and 1.9 μg.mL–1 for Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, respectively. These results were suggesting that the 0.5 % HERP membrane has the potential for future studies for wound application.
Permanent Link: http://hdl.handle.net/11104/0316936