Adenosine receptor modulates permissiveness of baculovirus (budded virus) infection via regulation of energy metabolism in Bombyx mori.

0524080 - BC 2021 RIV CH eng J - Journal Article
Lin, Yu-Hsien - Tai, C.-C. - Brož, Václav - Tang, C.-K. - Chen, P. - Wu, C. P. - Li, C.-H. - Wu, Y.-L.
Adenosine receptor modulates permissiveness of baculovirus (budded virus) infection via regulation of energy metabolism in Bombyx mori.
Frontiers in Immunology. Roč. 11, APR 28 (2020), č. článku 763. ISSN 1664-3224. E-ISSN 1664-3224
Grant - others:AV ČR(CZ) TWN-18-15; AV ČR(CZ) TWN-18-16
Institutional support: RVO:60077344
Keywords : glycolysis * baculovirus * adenosine signaling
OECD category: Biochemistry and molecular biology
Impact factor: 7.561, year: 2020
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fimmu.2020.00763/full

Although the modulation of host physiology has been interpreted as an essential process supporting baculovirus propagation, the requirement of energy supply for host antivirus reactions could not be ruled out. Our present study showed that metabolic induction upon AcMNPV (budded virus) infection of Bombyx mori stimulated virus clearance and production of the antivirus protein, gloverin. In addition, we demonstrated that adenosine receptor signaling (AdoR) played an important role in regulating such metabolic reprogramming upon baculovirus infection. By using a second lepidopteran model, Spodoptera frugiperda Sf-21 cells, we demonstrated that the glycolytic induction regulated by adenosine signaling was a conservative mechanism modulating the permissiveness of baculovirus infection. Another interesting finding in our present study is that both BmNPV and AcMNPV infection cause metabolic activation, but it appears that BmNPV infection moderates the level of ATP production, which is in contrast to a dramatic increase upon AcMNPV infection. We identified potential AdoR miRNAs induced by BmNPV infection and concluded that BmNPV may attempt to minimize metabolic activation by suppressing adenosine signaling and further decreasing the host's anti-baculovirus response. Our present study shows that activation of energy synthesis by adenosine signaling upon baculovirus infection is a host physiological response that is essential for supporting the innate immune response against infection.
Permanent Link: http://hdl.handle.net/11104/0315183