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Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera
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SYSNO ASEP 0523996 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera Author(s) Killer, Jiří (UZFG-Y) RID, ORCID
Mekadim, Chahrazed (UZFG-Y) ORCID
Bunešová, V. (CZ)
Mrázek, Jakub (UZFG-Y) RID, ORCID
Hroncová, Z. (CZ)
Vlková, E. (CZ)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 65, č. 1 (2020), s. 143-151Number of pages 9 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords bifidobacteria Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) QJ1510338 GA MZe - Ministry of Agriculture (MZe) NV16-27449A GA MZd - Ministry of Health (MZ) Method of publishing Limited access Institutional support UZFG-Y - RVO:67985904 UT WOS 000511921000013 EID SCOPUS 85065666745 DOI 10.1007/s12223-019-00716-0 Annotation The family Bifidobacteriaceae constitutes an important phylogenetic group that particularly includes bifidobacterial taxa demonstrating proven or debated positive effects on host health. The increasingly widespread application of probiotic cultures in the twenty-first century requires detailed classification to the level of particular strains. This study aimed to apply the glutamine synthetase class I (glnAI) gene region (717 bp representing approximately 50% of the entire gene sequence) using specific PCR primers for the classification, typing, and phylogenetic analysis of bifidobacteria and closely related scardovial genera. In the family Bifidobacteriaceae, this is the first report on the use of this gene for such purposes. To achieve high-value results, almost all valid Bifidobacteriaceae type strains (75) and 15 strains isolated from various environments were evaluated. The threshold value of the glnAI gene identity among Bifidobacterium species (86.9%) was comparable to that of other phylogenetic/identification markers proposed for bifidobacteria and was much lower compared to the 16S rRNA gene. Further statistical and phylogenetic analyses suggest that the glnAI gene can be applied as a novel genetic marker in the classification, genotyping, and phylogenetic analysis of isolates belonging to the family Bifidobacteriaceae. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2021 Electronic address https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=60666516332
Number of the records: 1