Number of the records: 1
Crucial Role of Microbiota in Experimental Psoriasis Revealed by a Gnotobiotic Mouse Model
- 1.
SYSNO ASEP 0523660 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Crucial Role of Microbiota in Experimental Psoriasis Revealed by a Gnotobiotic Mouse Model Author(s) Stehlíková, Z. (CZ)
Kostovčíková, Klára (UMG-J)
Kverka, Miloslav (UEM-P)
Rossmann, P. (CZ)
Dvorak, J. (CZ)
Novosádová, I. (CZ)
Kostovčík, M. (CZ)
Coufal, Š. (CZ)
Šrůtková, D. (CZ)
Procházková, P. (CZ)
Hudcovic, T. (CZ)
Kozáková, H. (CZ)
Stěpánková, R. (CZ)
Rob, F. (CZ)
Jůzlová, K. (CZ)
Hercogová, J. (CZ)
Tlaskalová-Hogenová, H. (CZ)
Zákostelská, Z. (CZ)Number of authors 18 Article number 236 Source Title Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 10, Feb 21 (2019)Number of pages 11 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords psoriasis ; antibiotics ; microbiota ; germ-free ; animal model ; imiquimod ; intestine ; skin Subject RIV EB - Genetics ; Molecular Biology OECD category Microbiology Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000459260100001 DOI 10.3389/fmicb.2019.00236 Annotation Psoriatic patients have altered microbiota, both in the intestine and on the skin. It is not clear, however, whether this is a cause or consequence of the disease. In this study, using an experimental mouse model of psoriasis induced by imiquimod (IMO), we show that oral treatment with a broad spectrum of antibiotics (MIX) or metronidazole (MET) alone mitigates the severity of skin inflammation through downregulation of Th17 immune response in conventional mice. Since some antibiotics, including MET, can influence immune system reactivity, we also evaluated the effect of MIX in the same model under germ-free (GF) conditions. GF mice treated with MET did not show milder signs of imiquimod-induced skin inflammation (IISI) which supports the conclusion that the therapeutic effect is mediated by changes in microbiota composition. Moreover, compared to controls, mice treated with MIX had a significantly higher abundance of the genus Lactobacillus in the intestine and on the skin. Mice treated with MET had a significantly higher abundance of the genera Bifidobacterium and Enterococcus both on the skin and in the intestine and of Parabacteroides distasonis in the intestine. Additionally, GF mice and mice monocolonized with either Lactobacillus plantarum or segmented filamentous bacteria (SFB) were more resistant to IISI than conventional mice. Interestingly, compared to GF mice, IMQ induced a higher degree of systemic Th17 activation in mice monocolonized with SFB but not with L. plantarum. The present findings provide evidence that intestinal and skin microbiota directly regulates IISI and emphasizes the importance of microbiota in the pathogenesis of psoriasis. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2020 Electronic address https://www.frontiersin.org/articles/10.3389/fmicb.2019.00236/full
Number of the records: 1