Cell wall contributes to the stability of plasma membrane nanodomain organization of Arabidopsis thaliana FLOTILLIN2 and HYPERSENSITIVE INDUCED REACTION1 proteins

Daněk, Michal; Angelini, J.; Malínská, Kateřina; Andrejch, J.; Amlerová, J.; Kocourková, Daniela; Brouzdová, Jitka; Valentová, O.; Martinec, Jan; Petrášek, Jan

doi:https://dx.doi.org/10.1111/tpj.14566

Number of the records: 1

Cell wall contributes to the stability of plasma membrane nanodomain organization of Arabidopsis thaliana FLOTILLIN2 and HYPERSENSITIVE INDUCED REACTION1 proteins

1.

SYSNO ASEP	0523575
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cell wall contributes to the stability of plasma membrane nanodomain organization of Arabidopsis thaliana FLOTILLIN2 and HYPERSENSITIVE INDUCED REACTION1 proteins
Author(s)	Daněk, Michal (UEB-Q)_ORCID Angelini, J. (CZ) Malínská, Kateřina (UEB-Q)_{RID, ORCID} Andrejch, J. (CZ) Amlerová, J. (CZ) Kocourková, Daniela (UEB-Q)_ORCID Brouzdová, Jitka (UEB-Q) Valentová, O. (CZ) Martinec, Jan (UEB-Q)_{RID, ORCID} Petrášek, Jan (UEB-Q)_{RID, ORCID}
Number of authors	10
Source Title	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 101, č. 3 (2020), s. 619-636
Number of pages	18 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Arabidopsis thaliana ; cell wall ; cytoskeleton ; flotillin ; hypersensitive induced reaction protein ; lateral mobility ; membrane nanodomains ; plasma membrane ; tonoplast
Subject RIV	CE - Biochemistry
OECD category	Biochemical research methods
R&D Projects	GA14-09685S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000495489000001
EID SCOPUS	85074863104
DOI	10.1111/tpj.14566
Annotation	Current models of plasma membrane (PM) postulate its organization in various nano- and micro-domains with distinct protein and lipid composition. While metazoan PM nanodomains usually display high lateral mobility, the dynamics of plant nanodomains is often highly spatially restricted. Here we have focused on the determination of the PM distribution in nanodomains for Arabidopsis thaliana flotillin (AtFLOT) and hypersensitive induced reaction proteins (AtHIR), previously shown to be involved in response to extracellular stimuli. Using in vivo laser scanning and spinning disc confocal microscopy in Arabidopsis thaliana we present here their nanodomain localization in various epidermal cell types. Fluorescence recovery after photobleaching (FRAP) and kymographic analysis revealed that PM-associated AtFLOTs contain significantly higher immobile fraction than AtHIRs. In addition, much lower immobile fractions have been found in tonoplast pool of AtHIR3. Although members of both groups of proteins were spatially restricted in their PM distribution by corrals co-aligning with microtubules (MTs), pharmacological treatments showed no or very low role of actin and microtubular cytoskeleton for clustering of AtFLOT and AtHIR into nanodomains. Finally, pharmacological alteration of cell wall (CW) synthesis and structure resulted in changes in lateral mobility of AtFLOT2 and AtHIR1. Accordingly, partial enzymatic CW removal increased the overall dynamics as well as individual nanodomain mobility of these two proteins. Such structural links to CW could play an important role in their correct positioning during PM communication with extracellular environment.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2020
Electronic address	http://doi.org/10.1111/tpj.14566

Number of the records: 1