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TATAA courses – 3 days Hands-on qPCR
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SYSNO ASEP 0523265 Document Type U - Organizing Conference, Workshop, Exhibition R&D Document Type W - Uspořádání workshopu R&D Document Type Není vybrán druh dokumentu Title TATAA courses – 3 days Hands-on qPCR Author(s) Kubista, Mikael (BTO-N) RID
Šindelka, Radek (BTO-N) RID
Valihrach, Lukáš (BTO-N) RID, ORCID
Švec, David (BTO-N) RID
Rohlová, Eva (BTO-N)
Smolík, Ondřej (BTO-N)
Franko, Filip (BTO-N)Event type W - Workshop Event date 08.04.2019 - 10.04.2019 VEvent location Vestec Country CZ - Czech Republic Event type WRD Total number of participants 9 Number of foreign participants 5 Language eng - English Keywords qPCR ; biostatistic ; reverese transcription Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation Day 1. Introduction to PCR and qPCR. qPCR data evaluation. Primer and probe design. Optimization of qPCR protocols. Hands-on lab running qPCR analyzing unknown samples with standard curve. Day 2. Normalization. Basic quantification theory. Absolute quantification strategies. Validation of assays. Quantification calculation examples. Hands-on lab running qPCR and calculating relative quantities with different strategies. Day 3. Sample preparation and isolation. Reverse transcription, RT. The MIQE guidelines. Group discussion Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2020
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