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Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries
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SYSNO ASEP 0522452 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries Author(s) Křenková, Jana (UIACH-O) RID, ORCID
Duša, Filip (UIACH-O) RID, ORCID
Čmelík, Richard (UIACH-O) RIDNumber of authors 3 Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 41, č. 9 (2020), s. 684-690Number of pages 7 s. Publication form Print - P Language eng - English Country DE - Germany Keywords hydrazone formation ; labeling ; oligosaccharides ; reductive amination Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA18-00062S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000513664800001 EID SCOPUS 85079716358 DOI 10.1002/elps.201900475 Annotation In this work, we compare labeling by two negatively charged fluorescent labels, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and 8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-
trisulfonic acid (Cascade Blue hydrazide [CBH]). Effectiveness of the labeling chemistries were investigated by 4-hydroxybenzaldehyde and maltoheptaose followed by LC/UV-MS
and CE/LIF analysis, respectively. The reaction yield of APTS labeling was determined to be only ∼10%. This is due to reduction of almost 90% of the analyte by sodium cyanoborohydride
to alcohol, which cannot be further labeled via reductive amination. However, the CBH labeling provides ∼90% reaction yield based on the LC/UV-MS measurements. The
significantly higher labeling yield was also confirmed by CE/LIF measurements. Finally, the more effective hydrazone formation technique of CBH was characterized and applied
for N-linked glycan analysis by CE/LIF.Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0306941
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