Number of the records: 1  

Vicinal Diol-Tethered Nucleobases as Targets for DNA Redox Labeling with Osmate Complexes

  1. 1.
    SYSNO ASEP0520341
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleVicinal Diol-Tethered Nucleobases as Targets for DNA Redox Labeling with Osmate Complexes
    Author(s) Havranová-Vidláková, Pavlína (BFU-R) ORCID
    Krömer, Matouš (UOCHB-X) RID, ORCID
    Sýkorová, Veronika (UOCHB-X) ORCID, RID
    Trefulka, Mojmír (BFU-R) RID, ORCID
    Fojta, Miroslav (BFU-R) RID, ORCID
    Havran, Luděk (BFU-R) RID, ORCID
    Hocek, Michal (UOCHB-X) RID, ORCID
    Number of authors7
    Source TitleChembiochem. - : Wiley - ISSN 1439-4227
    Roč. 21, 1/2 (2020), s. 171-180
    Number of pages11 s.
    Publication formPrint - P
    Languageeng - English
    CountryDE - Germany
    Keywordsnucleoside triphosphates ; electrochemistry ; inhibition ; DNA
    Subject RIVCE - Biochemistry
    OECD categoryBiochemistry and molecular biology
    Subject RIV - cooperationInstitute of Organic Chemistry and Biochemistry - Electrochemistry
    R&D ProjectsGA15-08434S GA ČR - Czech Science Foundation (CSF)
    EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707 ; UOCHB-X - RVO:61388963
    UT WOS000481172600001
    EID SCOPUS85070713153
    DOI10.1002/cbic.201900388
    AnnotationSix-valent osmium (osmate) complexes with nitrogenous ligands have previously been used for the modification and redox labeling of biomolecules involving vicinal diol moieties (typically, saccharides or RNA). In this work, aliphatic (3,4-dihydroxybutyl and 3,4-dihydroxybut-1-ynyl) or cyclic (6-oxo-6-(cis-3,4-dihydroxypyrrolidin-1-yl)hex-2-yn-1-yl, PDI) vicinal diols are attached to nucleobases to functionalize DNA for subsequent redox labeling with osmium(VI) complexes. The diol-linked 2 '-deoxyribonucleoside triphosphates were used for the polymerase synthesis of diol-linked DNA, which, upon treatment with K2OsO3 and bidentate nitrogen ligands, gave the desired Os-labeled DNA, which were characterized by means of the gel-shift assay and ESI-MS. Through ex situ square-wave voltammetry at a basal plane pyrolytic graphite electrode, the efficiency of modification/labeling of individual diols was evaluated. The results show that the cyclic cis-diol (PDI) was a better target for osmylation than that of the flexible aliphatic ones (alkyl- or alkynyl-linked). The osmate adduct-specific voltammetric signal obtained for Os-VI-treated DNA decorated with PDI showed good proportionality to the number of PDI per DNA molecule. The Os-VI reagents (unlike OsO4) do not attack nucleobases, thus offering specificity of modification on the introduced glycol targets.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2021
    Electronic addresshttps://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201900388
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.