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New data on spermiogenesis and trepaxonematan axoneme in basal tapeworms (Cestoda, Caryophyllidea, Lytocestidae) parasitizing cyprinid fishes
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SYSNO ASEP 0520177 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title New data on spermiogenesis and trepaxonematan axoneme in basal tapeworms (Cestoda, Caryophyllidea, Lytocestidae) parasitizing cyprinid fishes Author(s) Matoušková, M. (SK)
Bílý, Tomáš (BC-A) ORCID
Bruňanská, M. (SK)
Oros, M. (SK)
Kostič, B. (SK)
Nebesářová, Jana (BC-A) RID, ORCIDNumber of authors 6 Article number 12881 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 9, SEP 9 2019 (2019)Number of pages 11 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords glaridacris-catostomi cooper ; wenyonia-virilis woodland ; spermatological characters ; spermatozoon ultrastructure ; sperm ultrastructure ; 1st data ; platyhelminthes ; pallas ; spermatogenesis ; tomography Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000484657300005 EID SCOPUS 85071966986 DOI 10.1038/s41598-019-49312-9 Annotation Monozoic caryophyllidean cestodes, intestinal parasites of cyprinid fishes, represent a group of tapeworms with an unclear evolutionary history. As spermatology may provide phylogenetically important data, the spermiogenesis and ultrastructure of the mature spermatozoon have been investigated using an integrative approach combining transmission electron microscopy, cytochemistry and electron tomography in Khawia rossittensis (Szidat, 1937). The process of spermatid formation is accompanied by the presence of ultrastructural characters not described in traditional models of spermiogenesis, e.g., apical electron-dense material, the two striated roots situated unusually opposite each other, branching of typical striated roots, an intercentriolar body comprising five electron-dense and four electron-lucent layers, rotation of both free flagella and flagellar buds to the median cytoplasmic process at 90 degrees, and a complete proximodistal fusion. The synchronous rotation of both flagellar buds and growing free flagella is an evolutionarily linked pattern favouring the hypothesis that the Caryophyllidea are not ancestral but are secondarily derived from polyzoic forms. Electron tomography analysis has revealed a unique feature of two helicoidal tubular structures in the central electron-dense core of the axoneme of mature spermatozoon. These data provide new insights into the architecture of the 9 + '1' axoneme, which is shared by male gametes of all trepaxonematan Platyhelminthes. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://www.nature.com/articles/s41598-019-49312-9.pdf
Number of the records: 1