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Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking
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SYSNO ASEP 0518889 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking Author(s) Saurav, Kumar (MBU-M) ORCID
Macho, Markéta (MBU-M) ORCID
Kust, Andreja (MBU-M) ORCID
Delawská, Kateřina (MBU-M) ORCID
Hájek, Jan (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 64, č. 5 (2019), s. 645-654Number of pages 10 s. Language eng - English Country CZ - Czech Republic Keywords peptidic natural-products ; drug discovery ; database search Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GJ19-17868Y GA ČR - Czech Science Foundation (CSF) Research Infrastructure C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000496409500005 EID SCOPUS 85070227618 DOI 10.1007/s12223-019-00737-9 Annotation The rapid emergence of resistance in pathogenic bacteria together with a steep decline in economic incentives has rendered a new wave in the drug development by the pharmaceutical industry and researchers. Since cyanobacteria are recognized as wide producers of pharmaceutically important compounds, we investigated thirty-four cyanobacterial extracts prepared by solvents of different polarities for their antimicrobial potential. Almost all tested cyanobacterial strains exhibited some degree of antimicrobial bioactivity, with more general effect on fungal strains compared with bacteria. Surprisingly ~50% of cyanobacterial extracts exhibited specific activity against one or few bacterial indicator strains with Gram-positive bacteria being more affected. Extracts of two most promising strains were subjected to activity-guided fractionation and determination of the minimum inhibitory concentration (MIC) against selected bacterial and fungal isolates. Multiple fractions were responsible for their antimicrobial effect with MIC reaching low-micromolar concentrations and in some of them high level of specificity was recorded. Twenty-six bioactive fractions analyzed on LC-HRMS/MS and Global Natural Product Social Molecular Networking (GNPS) online workflow using dereplication resulted in identification of only forty-nine peptide spectrum matches (PSMs) with eleven unique metabolites spectrum matches (MSMs). Interestingly, only three fractions from Nostoc calcicola Lukesova 3/97 and four fractions from Desmonostoc sp. Cc2 showed the presence of unique MSMs suggesting the presence of unknown antimicrobial metabolites among majority of bioactive fractions from both the strains. Our results highlight potential for isolation and discovery of potential antimicrobial bioactive lead molecules from cyanobacterial extracts. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://link.springer.com/article/10.1007%2Fs12223-019-00737-9
Number of the records: 1