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The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study

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    SYSNO ASEP0518521
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleThe genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study
    Author(s) Novotná, Božena (UEM-P)
    Sikorová, Jitka (UEM-P)
    Milcová, Alena (UEM-P)
    Pechout, M. (CZ)
    Dittrich, L. (CZ)
    Vojtíšek-Lom, M. (CZ)
    Rössner ml., Pavel (UEM-P) RID, ORCID
    Brzicová, Táňa (UEM-P)
    Topinka, Jan (UEM-P) RID, ORCID
    Article numberUNSP 403034
    Source TitleMutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
    Roč. 845, SI (2019)
    Number of pages6 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsA549 cells ; biodiesel ; comet assay
    Subject RIVEB - Genetics ; Molecular Biology
    OECD categoryToxicology
    R&D ProjectsGA18-04719S GA ČR - Czech Science Foundation (CSF)
    LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    LM2015073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    EF16_013/0001821 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportUEM-P - RVO:68378041
    UT WOS000489193400014
    EID SCOPUS85063280432
    DOI10.1016/j.mrgentox.2019.03.007
    AnnotationAn analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load urban creep conditions, common for truck traffic in heavily congested urban areas, being one of the worst. We aimed to detect the genotoxicity of organic extracts from particulate matter in the exhaust of the diesel engine Zetor 1505 running on diesel and biodiesel (B100) fuels at characteristic modes of extended urban creep, typical for transit truck traffic in Prague, comparing the first 5 min of idling with extended (20-80 min) idling, full load after idle, stabilized full load, and 30% load. The diluted exhaust was sampled with high volume samplers on glass fiber fluorocarbon coated filters. The filters were extracted with dichloromethane and DNA damage was analyzed in A549 cells using comet assay, with the inclusion of formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (ENDOIII) to recognize oxidized DNA bases. The cells were exposed to extractable organic matter (EOM) for 4 and 24 h at non-cytotoxic dose corresponding to 0.001 m(3) of undiluted exhaust gas per ml cell media. At the 4 h exposure interval, all samples from B100 and diesel emissions induced DNA damage. EOM from the extended idle engine mode exerted the strongest genotoxic effect for both fuels. Twenty hours later, the cells exposed to diesel EOM exhibited a further increase of DNA strand breaks compared to the preceding interval. In contrast, DNA damage seemed to be fully repaired in cells treated with EOM derived from biodiesel B100. The preliminary results suggest that (i) diesel emissions are more genotoxic than the emissions from B100, (ii) biodiesel induced DNA lesions are repaired within 24 h.
    WorkplaceInstitute of Experimental Medicine
    ContactLenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
    Year of Publishing2020
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S1383571818303462?via%3Dihub
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