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The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study
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SYSNO ASEP 0518521 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study Author(s) Novotná, Božena (UEM-P)
Sikorová, Jitka (UEM-P)
Milcová, Alena (UEM-P)
Pechout, M. (CZ)
Dittrich, L. (CZ)
Vojtíšek-Lom, M. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCID
Brzicová, Táňa (UEM-P)
Topinka, Jan (UEM-P) RID, ORCIDArticle number UNSP 403034 Source Title Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
Roč. 845, SI (2019)Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords A549 cells ; biodiesel ; comet assay Subject RIV EB - Genetics ; Molecular Biology OECD category Toxicology R&D Projects GA18-04719S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001821 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEM-P - RVO:68378041 UT WOS 000489193400014 EID SCOPUS 85063280432 DOI 10.1016/j.mrgentox.2019.03.007 Annotation An analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load urban creep conditions, common for truck traffic in heavily congested urban areas, being one of the worst. We aimed to detect the genotoxicity of organic extracts from particulate matter in the exhaust of the diesel engine Zetor 1505 running on diesel and biodiesel (B100) fuels at characteristic modes of extended urban creep, typical for transit truck traffic in Prague, comparing the first 5 min of idling with extended (20-80 min) idling, full load after idle, stabilized full load, and 30% load. The diluted exhaust was sampled with high volume samplers on glass fiber fluorocarbon coated filters. The filters were extracted with dichloromethane and DNA damage was analyzed in A549 cells using comet assay, with the inclusion of formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (ENDOIII) to recognize oxidized DNA bases. The cells were exposed to extractable organic matter (EOM) for 4 and 24 h at non-cytotoxic dose corresponding to 0.001 m(3) of undiluted exhaust gas per ml cell media. At the 4 h exposure interval, all samples from B100 and diesel emissions induced DNA damage. EOM from the extended idle engine mode exerted the strongest genotoxic effect for both fuels. Twenty hours later, the cells exposed to diesel EOM exhibited a further increase of DNA strand breaks compared to the preceding interval. In contrast, DNA damage seemed to be fully repaired in cells treated with EOM derived from biodiesel B100. The preliminary results suggest that (i) diesel emissions are more genotoxic than the emissions from B100, (ii) biodiesel induced DNA lesions are repaired within 24 h. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S1383571818303462?via%3Dihub
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