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Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces
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SYSNO ASEP 0518314 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces Author(s) Hasoň, Stanislav (BFU-R) RID, ORCID
Pivoňková, Hana (BFU-R) ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 3 Article number 113364 Source Title Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 849, SEP 15 2019 (2019)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords single-stranded-dna ; interfacial behavior ; phase transients ; nucleic-acid ; electrochemical-behavior ; dipole-moments Subject RIV CG - Electrochemistry OECD category Analytical chemistry R&D Projects GA16-01625S GA ČR - Czech Science Foundation (CSF) EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000495482900018 DOI 10.1016/j.jelechem.2019.113364 Annotation Two-dimensional (2D) condensation of nucleic acid components at mercury electrodes around the potential of zero charge has been known for decades. In our previous pilot study, we reported on 2D condensation of homopyrimidine oligodeoxynucleotides (ODNs) at the hanging mercury drop electrode (HMDE) in negative potential region. In this work we focused in detail on the effect of the homopyrimidine ODN length (from 3- to 50-mers) on the formation and properties of 2D condensed films at the HMDE. To elicit possible orientation of the homopyrimidine ODN molecules in their 2D condensed layers (i.e. surface concentration of the ODN molecules and the cross-section areas per adsorbed ODN molecules) we used a model in which the maximum surface concentration of the adsorbed ODN molecules is determined from linear dependences of the square of capacitance pit width on temperature. Effect of the length of homoadenine ODNs on their adsorption/desorption processes were studied for comparison, as well as effects of ionic strength and base protonation. Further we focused on the effects of the length of periodically repeated homothymine/homocytosine stretches in 30-mer ODNs on their interfacial behavior at the negatively charged HMDE. Experiments with binary mixtures of noncomplementary and complementary 15-mer homo-ODNs revealed dominance of the homopyrimidine, particularly cytosine, ODN responses in such competitive arrangement. The usefulness of solid silver amalgam electrode for this type of studies is demonstrated. Finally, potential applications of our results in label-free electrochemical analysis of alternative DNA structures are briefly discussed. (C) 2019 Elsevier B.V. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S1572665719306320?via%3Dihub
Number of the records: 1