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Novel PCB-degrading Rhodococcus strains able to promote plant growth for assisted rhizoremediation of historically polluted soils
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SYSNO ASEP 0518271 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel PCB-degrading Rhodococcus strains able to promote plant growth for assisted rhizoremediation of historically polluted soils Author(s) Vergani, L. (IT)
Mapelli, F. (IT)
Šuman, J. (CZ)
Cajthaml, Tomáš (MBU-M) RID, ORCID
Uhlík, O. (CZ)
Borin, S. (IT)Article number e0221253 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 14, č. 8 (2019)Number of pages 17 s. Language eng - English Country US - United States Keywords polychlorinated-biphenyls pcbs ; contaminated soils ; bioremediation Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000485036900042 EID SCOPUS 85071122974 DOI 10.1371/journal.pone.0221253 Annotation Extended soil contamination by polychlorinated biphenyls (PCBs) represents a global environmental issue that can hardly be addressed with the conventional remediation treatments. Rhizoremediation is a sustainable alternative, exploiting plants to stimulate in situ the degradative bacterial communities naturally occurring in historically polluted areas. This approach can be enhanced by the use of bacterial strains that combine PCB degradation potential with the ability to promote plant and root development. With this aim, we established a collection of aerobic bacteria isolated from the soil of the highly PCB-polluted site 'SIN Brescia-Caffaro' (Italy) biostimulated by the plant Phalaris arundinacea. The strains, selected on biphenyl and plant secondary metabolites provided as unique carbon source, were largely dominated by Actinobacteria and a significant number showed traits of interest for remediation, harbouring genes homologous to bphA, involved in the PCB oxidation pathway, and displaying 2,3-catechol dioxygenase activity and emulsification properties. Several strains also showed the potential to alleviate plant stress through 1-aminocyclopropane-1-carboxylate deaminase activity. In particular, we identified three Rhodococcus strains able to degrade in vitro several PCB congeners and to promote lateral root emergence in the model plant Arabidopsis thaliana in vivo. In addition, these strains showed the capacity to colonize the root system and to increase the plant biomass in PCB contaminated soil, making them ideal candidates to sustain microbial-assisted PCB rhizoremediation through a bioaugmentation approach. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221253
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