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What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments
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SYSNO ASEP 0517409 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments Author(s) Sedláček, P. (CZ)
Slaninova, E. (CZ)
Enev, V. (CZ)
Koller, M. (CZ)
Nebesářová, Jana (BC-A) RID, ORCID
Marová, I. (CZ)
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)Number of authors 10 Source Title Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 103, č. 4 (2019), s. 1905-1917Number of pages 13 s. Publication form Print - P Language eng - English Country DE - Germany Keywords transform infrared-spectroscopy ; secondary structure ; ralstonia-eutropha ; granules ; proteins ; 3-hydroxybutyrate ; hydroxybutyrate ; crystallization ; synthase ; behavior ; Polyhydroxyalkanoates crystallization ; Intracellular granules ; Stress conditions Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Subject RIV - cooperation Institute of Scientific Instruments - Electronics ; Optoelectronics, Electrical Engineering R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-20697S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BC-A - RVO:60077344 ; UPT-D - RVO:68081731 UT WOS 000459250200029 EID SCOPUS 85059773078 DOI 10.1007/s00253-018-09584-z Annotation Polyhydroxyalkanoates (PHA) are storage polymers accumulated by numerous prokaryotes in form of intracellular granules. Native PHA granules are formed by amorphous polymer which reveals considerably higher elasticity and flexibility as compared to crystalline pure PHA polymers. The fact that bacteria store PHA in amorphous state has great biological consequences. It is not clear which mechanisms protect amorphous polymer in native granules from transition into thermodynamically favorable crystalline state. Here, we demonstrate that exposition of bacterial cells to particular stressors induces granules aggregation, which is the first but not sufficient condition for PHA crystallization. Crystallization of the polymer occurs only when the stressed bacterial cells are subsequently dried. The fact that both granules aggregation and cell drying must occur to induce crystallization of PHA indicates that both previously suggested hypotheses about mechanisms of stabilization of amorphous state of native PHA are valid and, in fact, both effects participate synergistically. It seems that the amorphous state of the polymer is stabilized kinetically by the low rate of crystallization in limited volume in small PHA granules and, moreover, water present in PHA granules seems to function as plasticizer protecting the polymer from crystallization, as confirmed experimentally for the first time by the present work. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://link.springer.com/article/10.1007%2Fs00253-018-09584-z
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