5-Methylcytosine-Rich Heterochromatin in Reptiles

0511986 - ÚŽFG 2020 RIV CH eng J - Journal Article
Schmid, M. - Steinlein, C. - Reiter, A. M. - Rovatsos, Michail - Altmanová, Marie - Mazzoleni, S. - Johnson Pokorná, Martina - Kratochvíl, L.
5-Methylcytosine-Rich Heterochromatin in Reptiles.
Cytogenetic and Genome Research. Roč. 157, 1-2 (2019), s. 53-64. ISSN 1424-8581. E-ISSN 1424-859X
R&D Projects: GA ČR GA17-22604S
Institutional support: RVO:67985904
Keywords : heterochromatin * hypermethylation * indirect immunofluorescence
OECD category: Zoology
Impact factor: 1.114, year: 2019
Method of publishing: Limited access
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=67857582765

An experimental approach using monoclonal anti-5-methylcytosine antibodies and indirect immunofluorescence was elaborated for detecting 5-methylcytosine-rich chromosome regions in reptilian chromosomes. This technique was applied to conventionally prepared mitotic metaphases of 2 turtle species and 12 squamate species from 8 families. The hypermethylation patterns were compared with C-banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and are located in constitutive heterochromatin. They are highly reproducible and often found in centromeric, pericentromeric, and interstitial positions of the chromosomes. Heterochromatic regions in differentiated sex chromosomes are particularly hypermethylated.
Permanent Link: http://hdl.handle.net/11104/0302215