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Chronopotentiometric Analysis of Proteins at Charged Electrode Surfaces
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SYSNO ASEP 0511687 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chronopotentiometric Analysis of Proteins at Charged Electrode Surfaces Author(s) Melnikova, E. (SK)
Izadi, Nasim (BFU-R) ORCID
Gál, M. (SK)
Ostatná, Veronika (BFU-R) RID, ORCIDNumber of authors 4 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 31, č. 10 (2019), s. 1868-1872Number of pages 5 s. Publication form Print - P Language eng - English Country DE - Germany Keywords bovine serum-albumin ; hydrogen evolution ; mercury ; polylysine ; catalysis ; Catalytic hydrogen evolution reaction Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000491476600004 DOI 10.1002/elan.201900239 Annotation Protein properties and functions are strongly dependent on the structure and amino acid content. In this work, catalytic hydrogen evolution reaction (CHER) of five proteins (human serum albumin, lysozyme, beta-synuclein, H2 A and H3 histones) were studied using constant current chronopotentiometric stripping (CPS) with the aim to find out the association between protein content and its electrochemical response. We have shown that the height and potential of CPS peak H in dependence on accumulation potential differed for the studied proteins, while the CPS peak area was almost the same for all of them. CV and CPS peaks H of Cys-containing proteins appeared at less negative potentials in comparison to proteins without Cys, suggesting easier CHER. Acidic and basic proteins not containing Cys can be also recognized due to their different CPS response after their adsorption at the positive and negative charged interface. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201900239
Number of the records: 1