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PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION
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SYSNO ASEP 0509660 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Transient gene expression as a tool to monitor and manipulate the levels of acidic phospholipids in plant cells Author(s) Noack, L. C. (FR)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Sekereš, Juraj (UEB-Q) RID, ORCID
Jaillais, Y. (FR)
Potocký, Martin (UEB-Q) RID, ORCIDNumber of authors 5 Source Title PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. - TOTOWA : HUMANA PRESS INC., 2019 / Cvrčková F. ; Žárský Viktor - ISSN 1064-3745 - ISBN 978-1-4939-9469-4 Pages s. 189-199 Number of pages 11 s. Number of copy 2400 Number of pages 380 Publication form Print - P Language eng - English Country US - United States Keywords Microscopy ; Nicotiana benthamiana ; Nicotiana tabacum ; Phosphoinositides ; Phospholipid-binding domains ; Pollen tube ; Transient expression Subject RIV ED - Physiology OECD category Cell biology R&D Projects GA17-27477S GA ČR - Czech Science Foundation (CSF) GC18-18290J GA ČR - Czech Science Foundation (CSF) GA19-21758S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000487932800013 EID SCOPUS 85066791632 DOI 10.1007/978-1-4939-9469-4_12 Annotation Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein–phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020
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