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PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION
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SYSNO ASEP 0509657 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Plant cell lines in cell morphogenesis research: From phenotyping to -Omics Author(s) Klíma, Petr (UEB-Q) RID, ORCID
Čermák, V. (CZ)
Srba, M. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCID
Šonka, J. (CZ)
Fischer, L. (CZ)
Opatrný, Z. (CZ)Number of authors 8 Source Title PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. - TOTOWA : HUMANA PRESS INC., 2019 / Cvrčková F. ; Žárský Viktor - ISSN 1064-3745 - ISBN 978-1-4939-9469-4 Pages roč. 1992 (2019), s. 367-376 Number of pages 10 s. Number of copy 2400 Number of pages 380 Publication form Print - P Language eng - English Country US - United States Keywords by-2 ; Cell lines ; Cell sorting ; Cloning ; Tobacco ; Transcriptomics ; Transformation ; vbi-0 Subject RIV ED - Physiology OECD category Biochemistry and molecular biology R&D Projects GA16-10948S GA ČR - Czech Science Foundation (CSF) GA16-19557S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000487932800026 EID SCOPUS 85066814721 DOI 10.1007/978-1-4939-9469-4_25 Annotation Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020
Number of the records: 1