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Electrochemical analysis of glycoprotein samples prepared on a pneumatically-controlled microfluidic device
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SYSNO ASEP 0508832 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrochemical analysis of glycoprotein samples prepared on a pneumatically-controlled microfluidic device Author(s) Novotný, Jakub (UIACH-O) ORCID
Ostatná, Veronika (BFU-R) RID, ORCID
Foret, František (UIACH-O) RID, ORCIDNumber of authors 3 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 31, č. 10 (2019), s. 1994-2000Number of pages 7 s. Publication form Print - P Language eng - English Country DE - Germany Keywords glycoprotein ; microfluidics ; square wave voltammetry Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Subject RIV - cooperation Institute of Biophysics - Electrochemistry R&D Projects GA18-18154S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 ; BFU-R - RVO:68081707 UT WOS 000491476600019 EID SCOPUS 85067518167 DOI 10.1002/elan.201900275 Annotation In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro-milling. The flow control and the fluid dosage into the micro-channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro-channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate-specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0299635
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